bs-0295M-Biotin [Conjugated Secondary Antibody]
Mouse Anti-Rabbit IgG Antibody (H+L), Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months..

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dong, Feng, et al. "The isolation and characterization of a telomerase immortalized goat trophoblast cell line." Placenta (2013).Read more>>
  • Mitsumata, Ryotaro, and Noriyuki Izutani. "METHOD FOR MEASURING HEMAGGLUTININ FROM INFLUENZA VIRUS." U.S. Patent No. 20,150,233,922. 20 Aug. 2015Read more>>
  • Wu, Shun‑Quan, et al. "miR-299-5p promotes cell growth and regulates G1/S transition by targeting p21Cip1/Waf1 in acute promyelocytic leukemia."Oncology Letters.Read more>>
  • Shao, Bo, et al. "Expression changes and significance of neurite outgrowth inhibitor A (Nogo-A), glial fibrillary acidic protein and insulin-like growth factor-1 in rat brain tissues after craniocerebral injury." Biomedical Research 28.1 (2017).Read more>>
  • Zhen, Zhen, et al. "DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-9.Read more>>
  • Wang et al. MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα. (2017) Exp.Ther.Me. 14:1706-1714Read more>>
  • Qu et al. MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1. (2017) Oncotarge. 8:52465-52473Read more>>
  • Ding et al. MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma. (2016) Oncol.Let. 11:1081-1088Read more>>
  • Wang et al. Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression. (2018) Mol.Med.Rep. 17:3511-3518Read more>>
  • Zhang et al. Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells. (2018) Exp.Ther.Med. 15:3908-3916Read more>>
  • Peng et al. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells. (2018) Oncol.Lett. 16:785-792Read more>>
  • Wang M et al. Protein 14-3-3ε Regulates Cell Proliferation and Casein Synthesis via PI3K-mTOR Pathway in Dairy Cow Mammary Epithelial Cells.(2018) J. Agric. Food Chem. Nov 14;66(45):12000-12008. Read more>>
  • Zhou J et al. Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS2 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation Neurochemical Research.2019.Read more>>
  • Bao XY et al. Transcriptome analysis revealed that aflatoxin M1 could cause cell cycle arrest in differentiated Caco-2 cells. Toxicol In Vitro. 2019 Mar 27;59:35-43. Read more>>
  • Wu C et al. Modulation of intestinal epithelial permeability and mucin mRNA (MUC2, MUC5AC, and MUC5B) expression and protein secretion in Caco-2/HT29-MTX co-cultures exposed to aflatoxin M1, ochratoxin A, and zearalenone individually or collectively.Toxicol Lett. 2019 Jul;309:1-9. Read more>>
  • Jiang S et al. Inhibitory Effect of Slit2-N on VEGF165-induced proliferation of vascular endothelia via Slit2-N-Robo4-Akt pathway in choroidal neovascularization. Cell Cycle. 2019 Jun;18(11):1241-1253. Read more>>
  • Tian M et al. A sensitive lateral flow immunochromatographic strip with Prussian blue nanoparticles mediated signal generation and cascade amplification. Sensors and Actuators: B. Chemical (2020).Read more>>
  • Wu L et al. Anti-toll-like receptor 2 antibody ameliorates hepatic injury, inflammation, fibrosis and steatosis in obesity-related metabolic disorder rats via regulating MAPK and NF-κB pathways. Int Immunopharmacol. 2020 Mar 6;82:106368. Read more>>
  • "Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224."Read more>>
  • Bai XS et al. MiR-195 alleviates ulcerative colitis in rats via MAPK signaling pathway. Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2640-2646. Read more>>
  • Ma Q et al. Pathological Mechanistic Studies of Osimertinib Resistance in Non-Small-Cell Lung Cancer Cells Using an Integrative Metabolomics-Proteomics Analysis. J Oncol. 2020 Mar 17;2020:6249829.Read more>>
  • Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res. 2020 Aug 7;19(8):3211-3219.Read more>>
  • Hong Cheet al. circ_0080145 Enhances Imatinib Resistance of Chronic Myeloid Leukemia by Regulating miR-326/PPFIA1 Axis. Cancer Biother Radiopharm. 2020 Jun 27.Read more>>
  • Jian Wanget al. NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression. BMC Cancer. 2020 Oct 6;20(1):966.Read more>>
  • Suleyman Arzimanet al. Salicylidene acylhydrazides attenuate survival of SH-SY5Y neuroblastoma cells through affecting mitotic regulator Speedy/RINGO and ERK/MAPKCPI3K/AKT signaling. Med Oncol. 2020 Jul 20;37(8):65.Read more>>
  • Huang S et al. Co?expression of tissue kallikrein?1 and tissue inhibitor of matrix metalloproteinase?1 improves myocardial ischemia?reperfusion injury by promoting angiogenesis and inhibiting oxidative stressMol Med Rep.2021 Feb;23(2):166.Read more>>
  • Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res. 2020 Aug 7;19(8):3211-3219.Read more>>
  • Xiaoli Gao. et al. Epigenetic upregulation of ssc-miR-124a following treatment with Clostridium perfringens beta2-toxin attenuates both apoptosis and inflammation in intestinal porcine epithelial cells. Arch Biochem Biophys. 2021 Apr;701:108806Read more>>
  • Huiqi Chen. et al. Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-B signalling in rats. Pharm Biol. 2021;59(1):121-128Read more>>
  • Jilong Han. et al. Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs. Plos One. 2021 Feb;16(2):e0246279Read more>>
  • Yan Liu. et al. CircTTBK2 Contributes to the Progression of Glioma Through Regulating miR-145-5p/CPEB4 Axis. Cancer Manag Res. 2020; 12: 8183C8195Read more>>
  • Hui Yuan. et al. Activation of calcium?sensing receptor?mediated autophagy in high glucose?induced cardiac fibrosis in?vitro. Mol Med Rep. 2020 Sep;22(3):2021-2031Read more>>
  • Lei Chen. et al. Circ_0032821 Facilitates Gastric Cancer Cell Proliferation, Migration, Invasion and Glycolysis by Regulating MiR-1236-3p/HMGB1 Axis. Cancer Manag Res. 2020; 12: 9965C9976Read more>>
  • Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184Read more>>
  • Yuebin Zheng. et al. Circ_0023028 contributes to the progression of laryngeal squamous cell carcinoma by upregulating LASP1 through miR-486-3p. 2021 Mar 23Read more>>
  • Zhusheng Huang. et al. Zoledronic AcidCGadolinium Coordination Polymer Nanorods for Improved Tumor Radioimmunotherapy by Synergetically Inducing Immunogenic Cell Death and Reprogramming the Immunosuppressive Microenvironment. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Liting Cheng. et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta Pharm Sin B. 2021 MayRead more>>
  • Sensen Lv. et al. LncRNA NEAT1 Knockdown Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Modulation of miR-182-5p/WISP1 Axis. 2021 May 27Read more>>
  • Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223Read more>>
  • Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692Read more>>
  • Chao Yang. et al. Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis. 2021 Jun 3Read more>>
  • Donghui Shen. et al. NF-B inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type. 2021 Jun 12Read more>>
  • Zhipeng Jiang. et al. EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway. J Exp Clin Canc Res. 2021 Dec;40(1):1-15Read more>>
  • Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077Read more>>
  • Wang Deng. et al. Alcohol inhibits alveolar fluid clearance through the epithelial sodium channel via the A2 adenosine receptor in acute lung injury. Mol Med Rep. 2021 Oct;24(4):1-13Read more>>
  • Wu, Guoxian. et al. Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12. World J Surg Oncol. 2021 Dec;19(1):1-12Read more>>
  • Hanyue Ying. et al. CircATL2 enhances paclitaxel resistance of ovarian cancer via impacting miR-506-3p/NFIB axis. 2021 Sep 19Read more>>
  • Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611C362Read more>>
  • Wu, Qingsi. et al. Targeted delivery of celastrol to glomerular endothelium and podocytes for chronic kidney disease treatment. Nano Res. 2021 Dec;:1-13Read more>>
  • Lulu Wang. et al. METTL3 is a key regulator of milk synthesis in mammary epithelial cells. 2021 Dec 11Read more>>