bs-0295P-A488 [Conjugated Isotype Control]
Rabbit IgG Isotype Control, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(Assay-dependent)
FCM(Assay-dependent)
IF(IHC-P)(Assay-dependent)
IF(IHC-F)(Assay-dependent)
IF(ICC)(Assay-dependent)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Zhi, et al. "Calpastatin Prevents NF-??B?CMediated Hyperactivation of Macrophages and Attenuates Colitis."he Journal of Immunology?(2013). Read more>>
  • Ma, Hongwei, et al. "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." ACS applied materials & interfaces 2.11 (2010): 3223-3230.Read more>>
  • Takigawa, Toshimichi, et al. "Glucocorticoid receptor-dependent immunomodulatory effect of ursodeoxycholic acid on liver lymphocytes in mice." American Journal of Physiology-Gastrointestinal and Liver Physiology (2013).Read more>>
  • Huang, Mo, et al. "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." Colloids and Surfaces B: Biointerfaces 85.1 (2011): 92-96.Read more>>
  • Lee, Ju Yeon, et al. "Molecular cloning and expression analysis of pig CD7." Veterinary Research Communications (2014): 1-7.Read more>>
  • Yu, Jie, et al. "Activation of liver X receptor enhances the proliferation and migration of endothelial progenitor cells and promotes vascular repair through PI3K/Akt/eNOS signaling pathway activation." Vascular Pharmacology (2014).Read more>>
  • Zhuang, Huiyu, et al. "Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells." Biochimie (2014).Read more>>
  • de Luna, Noemí, et al. "PYGM expression analysis in white blood cells: A complementary tool for diagnosing McARDLE disease?." Neuromuscular Disorders (2014).Read more>>
  • Brestoff, Jonathan R., et al. "Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity." Nature (2015).Read more>>
  • Wu, Qiong, et al. "A highly sensitive SPR biosensor based on a graphene oxide sheet modified with gold bipyramids, and its application to an immunoassay for rabbit IgG." Microchimica Acta (2015): 1-8.Read more>>
  • Fan, Kequan, et al. "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.Read more>>
  • Chau, David, et al. "Azacytidine sensitizes acute myeloid leukemia cells to arsenic trioxide by up-regulating the arsenic transporter aquaglyceroporin 9."Journal of Hematology & Oncology 8.1 (2015): 46.Read more>>
  • Wu, Yuanzi, Hongwei Ma, and Dayong Gu. "Quartz Crystal Microbalance as a tool for biomolecular interaction study." RSC Advances (2015).Read more>>
  • Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863Read more>>
  • Shang, Luoran, et al. "Photonic Crystal Microbubbles as Suspension Barcodes." Journal of the American Chemical Society (2016).Read more>>
  • Gaida, M. M., U. Dapunt, and G. M. Hänsch. "Sensing developing biofilms: the bitter receptor T2R38 on myeloid cells." Pathogens and Disease (2016): ftw004.Read more>>
  • DAVID, CHAU HAU WING. "POTENTIAL THERAPEUTIC APPLICATION OF ARSENIC TRIOXIDE IN ACUTE MYELOID LEUKAEMIA."Read more>>
  • DAVID, CHAU HAU WING. "POTENTIAL THERAPEUTIC APPLICATION OF ARSENIC TRIOXIDE IN ACUTE MYELOID LEUKAEMIA."Read more>>
  • Sente, Tahnee, et al. "Primary skeletal muscle myoblasts from chronic heart failure patients exhibit loss of anti-inflammatory and proliferative activity."BMC Cardiovascular Disorders 16.1 (2016): 1.Read more>>
  • Zhou, S. M., et al. "Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2 mutant of Haemophilus parasuis SC096 strain via the classical complement pathway." The Veterinary Journal 196.1 (2013): 111-113.Read more>>
  • Maurer, Susanne, et al. "Tasting Pseudomonas aeruginosa biofilms: human neutrophils express the bitter receptor T2R38 as sensor for the quorum sensing molecule N-(3-oxododecanoyl)-l-Homoserine Lactone." Frontiers in immunology 6 (2015).Read more>>
  • Benesch, Matthew GK, et al. "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." The FASEB Journal 29.9 (2015): 3990-4000.Read more>>
  • Benesch, Matthew GK, et al. "Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer." Endocrine-related cancer 22.4 (2015): 593-607.Read more>>
  • De Matteis, Giovanna, et al. "Evaluation of Leptin receptor expression on buffalo leukocytes." Veterinary Immunology and Immunopathology (2016).Read more>>
  • Kim JE, Yoo HJ, Gu JY, Kim HK (2016) Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation. PLoS ONE 11(6): e0156763.Read more>>
  • Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).Read more>>
  • Maurer, Susanne, et al. "Tasting Pseudomonas aeruginosa biofilms: human neutrophils express the bitter receptor T2R38 as sensor for the quorum sensing molecule N-(3-oxododecanoyl)-l-homoserine lactone." Frontiers in Immunology 6 (2015).Read more>>
  • Wang, Huimin, et al. "Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma." Oncotarget (2016).Read more>>
  • Iriyama, Noriyoshi, Yoshihiro Hatta, and Masami Takei. "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." Cancer Medicine (2016).Read more>>
  • Zhang, Jie, et al. "Role of PTX3 in corneal epithelial innate immunity against Aspergillus fumigatus infection." Experimental Eye Research (2016).Read more>>
  • Christensen, Anders, et al. "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." Oncotarget.Read more>>
  • Miyabe, Yoshishige, et al. "Complement C5a receptor is the key initiator of neutrophil adhesion igniting immune complex–induced arthritis." Science Immunology 2.7 (2017): eaaj2195.Read more>>
  • Jiang, Wen-Shuai, et al. "Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor." Sensors 17.2 (2017): 221.Read more>>
  • Jiang, Wen-Shuai, et al. "Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor." Sensors 17.2 (2017): 221.Read more>>
  • Chen, Baiwen, et al. "Effects of 1, 25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." International Immunopharmacology 47 (2017): 182-189.Read more>>
  • Jiang, Wen-Shuai, et al. "Reduced graphene oxide-based optical sensor for detecting specific protein." Sensors and Actuators B: Chemical (2017).Read more>>
  • Xia, Wenlong, and Jianwei Jiao. "Histone variant H3. 3 orchestrates neural stem cell differentiation in the developing brain." Cell Death & Differentiation (2017).Read more>>
  • Wang, Zhaojun, et al. "Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/-mice as well as smooth muscle cells." Vascular (2017): 1708538117713395.Read more>>
  • Zhao, Shuai, et al. "Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology." Proceedings of the National Academy of Sciences (2017): 201704155.Read more>>
  • Braun, Molly, et al. "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." Brain, Behavior, and Immunity (2017).Read more>>
  • Hao, Yingying, et al. "c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer." Oncology Reports.Read more>>
  • Shen, Tianjin, et al. "Brain-specific deletion of histone variant H2A. z results in cortical neurogenesis defects and neurodevelopmental disorder." Nucleic Acids Research.Read more>>
  • Deng et al. Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma. (2015) J.Exp.Clin.Cancer.Re. 34:96Read more>>
  • Cao et al. Leptin promotes migration and invasion of breast cancer cells by stimulating IL-8 production in M2 macrophages. (2016) Oncotarge. 7:65441-65453Read more>>
  • Wang et al. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. (2017) J.Diabetes.Res. 2017:1428537Read more>>
  • Huang et al. RANKL-induced M1 macrophages are involved in bone formation. (2017) Bone.Res. 5:17019Read more>>
  • Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234Read more>>
  • Forsberg et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. (2016) Elife. 5 Read more>>
  • Zhuang et al. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. (2014) Mol.Cancer. 13:243Read more>>
  • Shen et al. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. (2018) Nucleic.Acids.Res. 46:2290-2307Read more>>