bs-0296P-BF594 [Conjugated Isotype Control]
Mouse IgG Isotype Control, AbBy Fluor® 594 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Conjugation: AbBy Fluor® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(Assay-dependent)
FCM(Assay-dependent)
IF(IHC-P)(Assay-dependent)
IF(IHC-F)(Assay-dependent)
IF(ICC)(Assay-dependent)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Hua, et al. "Novel SPR biosensors based on metal nanoparticles decorated with graphene for immunoassay."?Sensors and Actuators B: Chemical?(2013).Read more>>
  • Lv, Zhenzhen, et al. "A simple and sensitive label-free fluorescent approach for protein detection based on A Perylene probe and aptamer." Biosensors and Bioelectronics (2014).Read more>>
  • Sun, Hukui, et al. "Non‐invasive imaging of allogeneic transplanted skin graft by 131I‐anti‐TLR5 mAb." Journal of Cellular and Molecular Medicine (2014).Read more>>
  • Wu, Qiong, et al. "A highly sensitive SPR biosensor based on a graphene oxide sheet modified with gold bipyramids, and its application to an immunoassay for rabbit IgG." Microchimica Acta (2015): 1-8.Read more>>
  • Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).Read more>>
  • Wu, Qiong, et al. "Hollow gold nanoparticle-enhanced SPR based sandwich immunoassay for human cardiac troponin I." Microchimica Acta (2017): 1-8.Read more>>
  • Wu, Qiong, et al. "Ultrasensitive magnetic field-assisted surface plasmon resonance immunoassay for human cardiac troponin I." Biosensors and Bioelectronics 96 (2017): 288-293.Read more>>
  • Zhang, Nan, et al. "Effect of vasopressin on hippocampal injury in a rodent model of asphyxial cardiopulmonary arrest." Experimental and therapeutic medicine 11.4 (2016): 1385-1392.Read more>>
  • Wang, Ning, et al. "A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2017).Read more>>
  • Wang, Ning, et al. "A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2017).Read more>>
  • Stepanova, Victoria, et al. "Urokinase-type plasminogen activator (uPA) is critical for progression of tuberous sclerosis complex 2 (TSC2)-deficient tumors." Journal of Biological Chemistry (2017): jbc-M117Read more>>
  • Liu et al. Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis. (2016) Mol.Med.Re. 13:1999-2006Read more>>
  • Deng et al. Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma. (2015) J.Exp.Clin.Cancer.Re. 34:96Read more>>
  • Peng et al. Ginsenoside Rg3 inhibits the senescence of prostate stromal cells through down-regulation of interleukin 8 expression. (2017) Oncotarget. 8:64779-64792Read more>>
  • Sun et al. Non-invasive imaging of allogeneic transplanted skin graft by 131I-anti-TLR5 mAb. (2014) J.Cell.Mol.Med. 18:2437-44Read more>>
  • He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78.Read more>>
  • Jia, W. et al. Elimination terminal fixed region screening and high-throughput kinetic determination of aptamer for lipocalin-1 by surface plasmon resonance imaging, (2018) Analytica Chimica ActaRead more>>
  • Chen X et al.Dual-digital encoded suspension array based on Raman spectroscopy and laser induced breakdown spectroscopy for multiplexed biodetection.(2019)Sensors and Actuators B: Chemical, 282, 457–468.Read more>>
  • Chen F et al. FeO@PDA immune probe-based signal amplification in surface plasmon resonance (SPR) biosensing of human cardiac troponin I. Colloids Surf B Biointerfaces 2019 Jan 28;177:105-111.Read more>>
  • Amamizu H et al. Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent-Induced Coronary Hyperconstricting Responses in Pigs In Vivo.Arterioscler Thromb Vasc Biol. 2019 Apr;39(4):741-753. Read more>>
  • Tao Y et al. Targeting of DDR1 with antibody-drug conjugates has antitumor effects in a mouse model of colon carcinoma. Mol Oncol. 2019 May 22.Read more>>
  • Peng T et al. The interaction of LOXL2 with GATA6 induces VEGFA expression and angiogenesis in cholangiocarcinoma. Int J Oncol. 2019 Jul 15. Read more>>
  • Wang B et al. Gold-nanorod-enhanced Raman spectroscopy encoded micro-quartz pieces for the multiplex detection of biomolecules. Anal Bioanal Chem. 2019 Jul 6.Read more>>
  • He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117.Read more>>
  • Chen X et al. Dual-wavelength digital holographic phase and fluorescence microscopy combining with Ramanspectroscopy for micro-quartz pieces-based dual-channel encoded suspension array. Opt Express. 2019 Feb 4;27(3):1894-1910.Read more>>
  • Liang Z et al. Aromatase-induced endogenous estrogen promotes tumour metastasis through estrogen receptor-α/matrix metalloproteinase 12 axis activation in castration-resistant prostate cancer. Cancer Lett. 2019 Sep 6. pii: S0304-3835(19)30465-3. Read more>>
  • Du S et al. A portable immune-thermometer assay based on the photothermal effect of graphene oxides for the rapid detection of Salmonella typhimurium. Biosens Bioelectron. 2019 Sep 6;144:111670.Read more>>
  • "Wang R et al. Expression of 5T4 extracellular domain fusion protein and preparation of anti-5T4 monoclonal antibody with high affinity and internalization efficiency.Protein Expr Purif. 2018 Jul 5. pii: S1046-5928(18)30212-2. "Read more>>
  • Dong Y et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects. Applied Materials Today 19 (2020) 100548.Read more>>
  • Jian-Jun Liet al. A highly specific and sensitive fluorescence quenching probe for carcinoembryonic antigen detection based on tetrapod Au nanostars with Ag coating. Materials Today Communications, 101373.Read more>>
  • Ningfei Guoet al. Lung adenocarcinoma\related TNF\\dependent inflammation upregulates MHC\II on alveolar type II cells through CXCR\2 to contribute to Treg expansion. FASEB J. 2020 Sep;34(9):12197-12213.Read more>>
  • Jian Zhouet al. Clinical significance of CD38 and CD101 expression in PD?1+CD8+ T?cells in patients with epithelial ovarian cancer. Oncol Lett. 2020 Jul;20(1):724-732.Read more>>
  • Nan Li. et al. A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers. Micromachines-Basel. 2021 Apr;12(4):347Read more>>
  • Xin Liu. et al. Estrogen inhibits bladder overactivity in rats with cyclophosphamide-induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells. 2021 Oct 07Read more>>
  • Sun Yimeng. et al. Mitophagy Protects the Retina Against Anti-Vascular Endothelial Growth Factor Therapy-Driven Hypoxia via Hypoxia-Inducible Factor-1 Signaling. Front Cell Dev Biol. 2021 Nov;0:3048Read more>>
  • Yue Wen. et al. TNF--dependent lung inflammation upregulates PD-L1 in monocyte-derived macrophages to contribute to lung tumorigenesis. FASEB J. 2022 Oct;36(11):e22595Read more>>
  • Nie Xin. et al. ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer. BMC CANCER. 2024 Dec;24(1):1-18Read more>>