bs-0296R-HRP [Conjugated Secondary Antibody]
Rabbit Anti-Mouse IgG Antibody (H+L), HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Munzer, D. G., et al. "Developing a Cyclin Blueprint as a Tool for Mapping the Cell Cycle in GS-NS0." Biochemical Engineering Journal (2013).Read more>>
  • Li, Wenliang, et al. "Development and partial validation of a recombinant E2-based indirect ELISA for detection of specific IgM antibody responses against classical swine fever virus." Journal of Virological Methods (2013).Read more>>
  • Wang, Beibei, et al. "Chlamydia pneumoniae Infection Promotes Vascular Smooth Muscle Cell Migration through a Toll-Like Receptor 2-Related Signaling Pathway." Infection and immunity 81.12 (2013): 4583-4591.Read more>>
  • Liao, Ruyi, et al. "Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis."PLOS ONE 10.7 (2015): e0132724.Read more>>
  • Yu, Linpo, Yang Liu, and Ming Zhou. "Improved electrochemiluminescence labels for heterogeneous microbead immunoassay." Analytical and Bioanalytical Chemistry (2016): 1-9.Read more>>
  • Wu, Shun‑Quan, et al. "miR-299-5p promotes cell growth and regulates G1/S transition by targeting p21Cip1/Waf1 in acute promyelocytic leukemia."Oncology Letters.Read more>>
  • Zhou, Qing, et al. "Construction of corneal epithelium with human amniotic epithelial cells and repair of limbal deficiency in rabbit models." Human cell28.1 (2015): 22-36.Read more>>
  • Cetintas, Vildan Bozok, et al. "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." Medicine 95.43 (2016): e5150.Read more>>
  • Yang, Wen-Tao, et al. "Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice." Journal of Veterinary Science (2017)18.2: 159-167.Read more>>
  • Zhang,et al.The Role of Tumor-Associated Ingredients in Serum on Antitumor Immunity.(2017) Drug Design Development and Therapy. 1:.Read more>>
  • Xia, P. et al. Mulberrin (Mul) reduces spinal cord injury (SCI)-induced apoptosis, inflammation and oxidative stress in rats via miroRNA-337 by targeting Nrf-2. (2018) Biomedicine & Pharmacotherapy, 107, 1480–1487. Read more>>
  • Luan Z et al. Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro. In Vitro Cell Dev Biol Anim. 2019 Aug 19. Read more>>
  • CJ Feng et al. Intra-Arterial Injection of Human Adipose-Derived Stem Cells Improves Viability of the Random Component of Axial Skin Flaps in Nude Mice. Journal of Plastic, Reconstructive & Aesthetic Surgery. 2019 Oct 10.Read more>>
  • Zhen H et al. The planarian Vinculin is required for the regeneration of GABAergic neurons in Dugesia japonica. Exp Cell Res. 2019 Jul 29:111540. Read more>>
  • Zhang JT et al. CRISPR/Cas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFα/CHX-induced Apoptosis. BioMed Research International.2019, Article ID 9071297.Read more>>
  • Dingmei Zhang. et al. Prenatal dexamethasone exposure caused fetal rats liver dysplasia by inhibiting autophagy-mediated cell proliferation. Toxicology. 2021 Feb;449:152664Read more>>
  • Hong-He Xiao. et al. Icarisid II promotes proliferation and neuronal differentiation of neural stem cells via activating Wnt/\catenin signaling pathway. 2021 Jan 17Read more>>
  • Hangyuan He. et al. Vaspin regulated cartilage cholesterol metabolism through miR155/LXR and participated in the occurrence of osteoarthritis in rats. Life Sci. 2021 Mar;269:119096Read more>>
  • Qing Lin. et al. 4?Methoxybenzylalcohol protects brain microvascular endothelial cells against oxygen?glucose deprivation/reperfusion?induced injury via activation of the PI3K/AKT signaling pathway. Exp Ther Med. 2021 Mar;21(3):1-1Read more>>
  • Di Tu Qiu. et al. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3K/AKT/mTOR and Nrf2/HO-1 Signaling Pathways. Biomed Res Int. 2020;2020:7069052Read more>>
  • Yongping Liu. et al. Elevated MicroRNA-326 Levels Regulate the IL-23/IL-23R/Th17 Cell Axis in Hashimoto's Thyroiditis by Targeting a Disintegrin and Metalloprotease 17. Thyroid. 2020 Sep;30(9):1327-1337Read more>>
  • Hui Yuan. et al. Activation of calcium?sensing receptor?mediated autophagy in high glucose?induced cardiac fibrosis in?vitro. Mol Med Rep. 2020 Sep;22(3):2021-2031Read more>>
  • Yan Liu. et al. Evaluation of a cystatin-like protein of Trichinella spiralis for serodiagnosis and identification of immunodominant epitopes using monoclonal antibodies. Vet Parasitol. 2020 May;:109127Read more>>
  • Fuyan Wang. et al. Decellularized porcine cornea-derived hydrogels for the regeneration of epithelium and stroma in focal corneal defects. Ocul Surf. 2020 Oct;18:748Read more>>
  • Xiao-Yi Gao. et al. Development of Fc-specific multi-biotinylated antibodies via photoreactive tandem AviTag repeats for the ultrasensitive determination of ochratoxin A. Food Control. 2022 Feb;132:108525Read more>>
  • Yunzhang Wu. et al. Deficiency of CARMA3 attenuates the development of bleomycin induced pulmonary fibrosis. Biochem Bioph Res Co. 2021 Dec;581:81Read more>>
  • Shuang Li. et al. Surface-Functionalized Silica-Coated Calcium Phosphate Nanoparticles Efficiently Deliver DNA-Based HIV-1 Trimeric Envelope Vaccines against HIV-1. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Jilong Zhou. et al. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation. 2022 Mar 2Read more>>
  • Yufei Liu. et al. Inflammation-Stimulated MSC-Derived Small Extracellular Vesicle miR-27b-3p Regulates Macrophages by Targeting CSF-1 to Promote Temporomandibular Joint Condylar Regeneration. 2022 Mar 11Read more>>
  • Hainan He. et al. Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation. AUTOPHAGY. 2022 Apr 25Read more>>
  • Yang, Rui-Cheng. et al. SARS-CoV-2 productively infects human brain microvascular endothelial cells. J NEUROINFLAMM. 2022 Dec;19(1):1-17Read more>>
  • Ya Sun. et al. Expression and hormone regulation of UCP2 in goat uterus. ANIM REPROD SCI. 2022 Aug;243:107015Read more>>
  • Jiayi Pan. et al. A Novel Therapeutic Tumor Vaccine Targeting MUC1 in Combination with PD-L1 Elicits Specific Anti-Tumor Immunity in Mice. VACCINES-BASEL. 2022 Jul;10(7):1092Read more>>
  • Hui Zhen. et al. The feedback loop between calcineurin, calmodulin-dependent protein kinase II, and nuclear factor of activated T-cells regulates the number of GABAergic neurons during planarian head regeneration.. FRONT MOL NEUROSCI. 2022 Sep;15:988803-988803Read more>>
  • Helei Li. et al. Matrix Regeneration Ability In Situ Induced by a Silk Fibroin Small-Caliber Artificial Blood Vessel In Vivo. POLYMERS-BASEL. 2022 Jan;14(18):3754Read more>>
  • Ning Zhang. et al. Spatial transcriptomics shows moxibustion promotes hippocampus astrocyte and neuron interaction. LIFE SCI. 2022 Oct;:121052Read more>>
  • Ting Lin. et al. CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells. INT J MOL SCI. 2022 Jan;23(19):11822Read more>>