bs-0297G-HRP [Conjugated Secondary Antibody]
Goat Anti-Human IgG Antibody (H+L), HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Goat

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at 4°C for 12 months. Store at 4°C for 12 months.

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:500-2000)
IHC-P(1:500-2000)
IHC-F(1:500-2000)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu, Lei, et al. "A SERS-based immunoassay with highly increased sensitivity using gold/silver core-shell nanorods." Biosensors and Bioelectronics (2012).Read more>>
  • Tan, Yuyu, et al. "Proximity-dependent Protein Detection Based on Enzyme-Assisted Fluorescence Signal Amplification." Biosensors and Bioelectronics (2013).Read more>>
  • Dong, Peipei, et al. "Ultrathin Gold-Shell Coated Silver Nanoparticles onto a Glass Platform for Improvement of Plasmonic Sensors." ACS Applied Materials & Interfaces (2013).Read more>>
  • Wang, Leilei, et al. "A C1q Domain Containing Protein from Scallop Chlamys farreri Serving as Pattern Recognition Receptor with Heat-Aggregated IgG Binding Activity." PloS one 7.8 (2012): e43289.Read more>>
  • Wang, Leilei, et al. "A C1q Domain Containing Protein from Scallop Chlamys farreri Serving as Pattern Recognition Receptor with Heat-Aggregated IgG Binding Activity." PloS one 7.8 (2012): e43289.Read more>>
  • Hao, Nan, et al. "An electrochemical immunosensing method based on silver nanoparticles." Journal of Electroanalytical Chemistry 656.1 (2011): 50-54.Read more>>
  • Tian, Lifang, et al. ʺAutoimmune and inflammatory responses in Kashin–Beck disease compared with rheumatoid arthritis and osteoarthritis.ʺ Human Immunology 72.10 (2011Read more>>
  • Zhang, Ruohu, et al. "Immunoassays based on surface-enhanced fluorescence using gap-plasmon-tunable Ag bilayer nanoparticle films." Journal of fluorescence 23.1 (2013): 71-77.Read more>>
  • Zhang, Ruohu, et al. "A Straightforward Immunoassay Applicable to a Wide Range of Antibodies Based on Surface Enhanced Fluorescence." Journal of Fluorescence (2013): 1-9.Read more>>
  • Zhang, Ruohu, et al. "Immunoassays based on surface-enhanced fluorescence using gap-plasmon-tunable Ag bilayer nanoparticle films." Journal of fluorescence 23.1 (2013): 71-77.Read more>>
  • Dai, Xiaoyan, et al. "Influence of the physical and chemical properties of magnetic nanoparticles on their performance in a chemiluminescence immunoassay." Clinical Biochemistry (2013).Read more>>
  • Xun, Yiping, et al. "Identification of prohibitin as an antigen in Behcet’s disease." Biochemical and Biophysical Research Communications (2014).Read more>>
  • Gao, Zhan, et al. "An amphioxus gC1q protein binds human IgG and initiates the classical pathway: Implications for a C1q‐mediated complement system in the basal chordate." European Journal of Immunology (2014).Read more>>
  • Zhao, Rui, et al. "Trace level detections of abrin with high SNR piezoresitive cantilever biosensor." Sensors and Actuators B: Chemical (2015).Read more>>
  • Du H, Shi L, Chen P, Yang W, Xun Y, Yang C, et al. (2015) Prohibitin Is Involved in Patients with IgG4 Related Disease. PLoS ONE 10(5): e0125331.Read more>>
  • Fan, Kequan, et al. "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.Read more>>
  • Wu, Lei, et al. "A reusable biosensor chip for SERS-fluorescence dual mode immunoassay." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.Read more>>
  • Wang, Chongwen, et al. "Polyethylenimine-interlayered silver-shell magnetic-core microspheres as a multifunctional SERS substrate." Journal of Materials Chemistry C (2015).Read more>>
  • Zhang, Si, et al. "A double signal electrochemical Human immunoglobulin G immunosensor based on gold nanoparticles-polydopamine functionalized reduced graphene oxide as a sensor platform and AgNPs/Carbon nanocomposite as signal probe and catalytic substrate." Biosensors and Bioelectronics (2015).Read more>>
  • Zhou, Peifu, et al. "CHARACTERIZATION OF A TUBERCULOSIS PATIENT'S SERA REACTIVE MYCOBACTERIUM TUBERCULOSIS TRANSCRIPTION FACTOR RV2175C." Journal of Immunoassay and Immunochemistry 35.2 (2014): 173-182.Read more>>
  • Liu L et al. Exosomal cyclophilin A as a novel noninvasive biomarker for Epstein-Barr virus associated nasopharyngeal carcinoma. Cancer Med. 2019 Jun;8(6):3142-3151. Read more>>
  • He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117.Read more>>
  • Kong LX et al. Lab-on-Tip: Protruding-shaped All-Fiber Plasmonic Microtip Probe Toward In-situ Chem-Bio Detection. Sensors and Actuators B: Chemical,2019 Dec12, 301:127128.Read more>>
  • Chen X et al. Hydrogel-based microbeads for Raman-encoded suspension array using the reversed-phase suspension polymerization method and ultraviolet light curing. Anal Bioanal Chem. 2020 May;412(12):2731-2741. Read more>>
  • He M et al. A Femtoliter On-Demand Droplet Dispensing System Based on Gigahertz Acoustic Resonator.Lab Chip. 2018 Aug 21;18(17):2540-2546.Read more>>
  • Zhou Y et al. Identification of a novel anti-heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease. Molecular Medicine REPORTS 21: 1771-1778, 2020.Read more>>
  • Zhou Y et al. Identification of a novel anti-heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease. Molecular Medicine REPORTS 21: 1771-1778, 2020.Read more>>
  • Zhang Y et al. Low Level Antibodies Against Alpha-Tropomyosin Are Associated With Increased Risk of Coronary Heart Disease. Front Pharmacol. 2020 Feb 27;11:195. Read more>>