bs-0297R-HRP [Conjugated Secondary Antibody]
Rabbit Anti-Human IgG Antibody (H+L), HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:500-2000)
IHC-P(1:500-2000)
IHC-F(1:500-2000)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tan, Yuyu, et al. "Proximity-dependent Protein Detection Based on Enzyme-Assisted Fluorescence Signal Amplification." Biosensors and Bioelectronics (2013).Read more>>
  • Wang, Liying, et al. "Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film." Talanta 78.1 (2009): 265-269.Read more>>
  • Li, Rui, et al. "4–Amino–1–(3–mercapto–propyl)–pyridine hexafluorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement." Analytical Chemistry (2014).Read more>>
  • Li, Rui, et al. "4–Amino–1–(3–mercapto–propyl)–pyridine hexafluorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement." Analytical Chemistry (2014).Read more>>
  • Zhang, Si, et al. "A double signal electrochemical Human immunoglobulin G immunosensor based on gold nanoparticles-polydopamine functionalized reduced graphene oxide as a sensor platform and AgNPs/Carbon nanocomposite as signal probe and catalytic substrate." Biosensors and Bioelectronics (2015).Read more>>
  • Wang et al. Preparation of colloidal gold immunochromatographic strip for detection of Paragonimiasis skrjabini. (2014) PLoS.One. 9:e92034Read more>>
  • Wang,et al.Vasoactive intestinal peptide inhibits airway smooth muscle cell proliferation in a mouse model of asthma via the ERK1/2 signaling pathway.(2018) Experimental Cell Research. 364:168-174.Read more>>
  • Wang H et al. CD137 ligand feedback upregulates PD‐L1 expression on lung cancer via T cell production of IFN‐γ. Thorac Cancer. 2019 Oct 17. Read more>>