bs-0301R [Primary Antibody]
Dnmt3 Beta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Dnmt3 Beta

Immunogen Range: 1-80/853


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1789

Swiss Prot: Q9UBC3

Source: KLH conjugated synthetic peptide derived from human Dnmt3 Beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 94


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Qian, et al. "Prenatal Cocaine Exposure Impairs Cognitive Function of Progeny Via Insulin Growth Factor II Epigenetic Regulation." Neurobiology of Disease (2015).Read more>>
  • Wu, Yuting, et al. "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." Toxicology and Applied Pharmacology 315 (2017): 35-49.Read more>>
  • Huang et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer. (2016) Oncol.Rep. 36:2705-2714Read more>>
  • Xiao-Tan Zhang. et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347Read more>>
  • Ai Wei. et al. Inhibition of DNA methylation derepresses PPAR and attenuates pulmonary fibrosis. 2021 Aug 11Read more>>
  • Chen Li. et al. Mixed-Lineage Leukemia 1 Inhibition Enhances the Differentiation Potential of Bovine Embryonic Stem Cells by Increasing H3K4 Mono-Methylation at Active Promoters. INT J MOL SCI. 2023 Jan;24(15):11901Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: human colon cancer tissue labeled with Anti-Dnmt-3-beta Polyclonal Antibody, Unconjugated (bs-0301R) at 1:600, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded: rat spinal cord tissue labeled with Anti-Dnmt-3-beta Polyclonal Antibody (bs-0301R), Unconjugated at 1:600, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Dnmt3 Beta Polyclonal Antibody, Unconjugated (bs-0301R) at 1:200 overnight at 4°C, DAB staining.


HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Dnmt3b) polyclonal Antibody, Unconjugated (bs-0301R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.