bs-0309R-HRP [Conjugated Secondary Antibody]
Rabbit Anti-Pig IgG Antibody (H+L), HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Fang, et al. "Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum." Talanta (2011).Read more>>
  • Li, XuePu, et al. "Sensitive immunoassay for porcine pseudorabies antibody based on fluorescence signal amplification induced by cation exchange in CdSe nanocrystals." Microchimica Acta 180.3-4 (2013): 303-310.Read more>>
  • Li, XuePu, et al. "Sensitive immunoassay for porcine pseudorabies antibody based on fluorescence signal amplification induced by cation exchange in CdSe nanocrystals." Microchimica Acta 180.3-4 (2013): 303-310.Read more>>
  • Li, Wenliang, et al. "Development and partial validation of a recombinant E2-based indirect ELISA for detection of specific IgM antibody responses against classical swine fever virus." Journal of Virological Methods (2013).Read more>>
  • LI, HELIN, et al. "Co-expression of the C-terminal domain of Yersinia enterocolitica invasin enhances the efficacy of classical swine-fever-vectored vaccine based on human adenovirus." Journal of Biosciences 40.1 (2015): 1-13.Read more>>
  • Li, Helin, Rui Gao, and Yanming Zhang. "A Promising Trigene Recombinant Human Adenovirus Vaccine Against Classical Swine Fever Virus." Viral Immunology (2016).Read more>>
  • Chen, Xiaohong, et al. "Secreted expression of truncated capsid protein from porcine circovirus type 2 in Pichia pastoris." Biotechnology Letters: 1-9.Read more>>
  • Wei, Bo, et al. "Magnetic beads-based enzymatic spectrofluorometric assay for rapid and sensitive detection of antibody against ApxIVA of Actinobacillus pleuropneumoniae." Biosensors and Bioelectronics 35.1 (2012): 390-393.Read more>>
  • Liu et al. Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology. (2015) Vet.Microbiol. 176:109-19Read more>>
  • Zhang H et al. Construction and immunological evaluation of recombinant Newcastle disease virus vaccines expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP3/GP5 proteins in pigs. Veterinary Microbiology,2019 108490. Read more>>
  • Yu Tao. et al. Immune responses induced by a combined vaccination with a recombinant chimera of Mycoplasma hyopneumoniae antigens and capsid virus-like particles of porcine circovirus type 2. Bmc Vet Res. 2020 Dec;16(1):1-13Read more>>
  • Yan Liu. et al. Evaluation of a cystatin-like protein of Trichinella spiralis for serodiagnosis and identification of immunodominant epitopes using monoclonal antibodies. Vet Parasitol. 2020 May;:109127Read more>>
  • Lijun Zhang. et al. A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection Against Actinobacillus pleuropneumoniae Infection in Mice and Pigs. FRONT VET SCI. 2022; 9: 902497Read more>>
  • Tongsheng Qi. et al. Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan Plateau. FRONT VET SCI. 2022 Jul 19;9:95338Read more>>
  • Jinchao Zhang. et al. Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau. ANIMALS. 2022 Jan;12(19):2723Read more>>