bs-0380R [Primary Antibody]
MBP Polyclonal Antibody
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Host: Rabbit

Target Protein: MBP

Immunogen Range: 69-85/167


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from Guinea Pig MBP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Oligodendrocyte Marker The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:1000-5000)
IHC-P(1:500-2000)
IHC-F(1:500-2000)
IF(1:500-2000)

Predicted Molecular Weight: 33


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Jia, et al. "Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats." Journal of the neurological sciences 325.1 (2013): 127-136.Read more>>
  • Gao, Yuhua, et al. "Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain." International Journal of Molecular Sciences 16.3 (2015): 5990-5999.Read more>>
  • Mori, Miki, et al. "Stromal Cell-Derived Factor-1α Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." International Journal of Molecular Sciences 16.8 (2015): 18018-18032.Read more>>
  • Luo, Guangying, et al. "Paternal bisphenol a diet changes prefrontal cortex proteome and provokes behavioral dysfunction in male offspring." Chemosphere (2017).Read more>>
  • Zhang et al. MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells. (2017) Exp.Ther.Med. 14:2809-2816Read more>>
  • Liang Z et al. A simple electrical stimulation cell culture system on the myelination of dorsal root ganglia and Schwann cells. Biotechniques. 2019 May 24. Read more>>
  • Haolu Sun. et al. iRhom1 rescues cognitive dysfunction in multiple sclerosis via preventing myelin injury. Genes Brain Behav. 2021 Nov;20(8):e12771Read more>>
  • Yuanxin Zhai. et al. High-efficiency Brain-targeted Intranasal Delivery of BDNF Mediated by Engineered Exosomes to Promote Remyelination. BIOMATER SCI-UK. 2022 AugRead more>>
  • Ayaka Nakatani. et al. S100A8 enhances IL-1 production from nasal epithelial cells in eosinophilic chronic rhinosinusitis. ALLERGOL INT. 2022 SepRead more>>
  • Yueqi Yang. et al. DHA and EPA Alleviate Epileptic Depression in PTZ-Treated Young Mice Model by Inhibiting Neuroinflammation through Regulating Microglial M2 Polarization and Improving Mitochondrial Metabolism. ANTIOXIDANTS-BASEL. 2023 Dec;12(12):2079Read more>>
  • Tomruk Canberk. et al. Effects of apelin on neonatal brain neurogenesis in L-NAME-induced maternal preeclampsia. SCI REP-UK. 2024 Aug;14(1):1-17Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-0380R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-0380R) at 1:200 overnight at 4°C, DAB staining.