Host: Rabbit
Target Protein: CRF
Immunogen Range: 145-185/196
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1392
Swiss Prot: P06850
Source: KLH conjugated synthetic peptide derived from human CRF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 4_21_
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Zoratto, F. et al.Intranasal oxytocin administration promotes emotional contagion and reduces aggression in a mouse model of callousness. (2018) Neuropharmacology.Sep 10;143:250-267. Read more>>
- Wang H et al. Leukemia inhibitory factor induces corticotropin-releasing hormone in mouse trophoblast stem cells. Biochem Biophys Res Commun. 2019 Nov 15. pii: S0006-291X(19)32179-5. Read more>>
- Lola Torz. et al. NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model. Int J Mol Sci. 2022 Jan;23(6):326Read more>>
Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody (bs-0382R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37°C DAPI(5ug/ml, blue) was used to stain the cell nuclei
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary and DAPI staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200 overnight at 4°C, DAB staining.