bs-0382R-HRP [Conjugated Primary Antibody]
CRF Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CRF

Immunogen Range: 145-185/196

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1392

Swiss Prot: P06850

Source: KLH conjugated synthetic peptide derived from human CRF

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin.

Storage Condition: Store at 4°C for 12 months.

Background: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Pig
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zoratto, F. et al.Intranasal oxytocin administration promotes emotional contagion and reduces aggression in a mouse model of callousness. (2018) Neuropharmacology.Sep 10;143:250-267. Read more>>
  • Wang H et al. Leukemia inhibitory factor induces corticotropin-releasing hormone in mouse trophoblast stem cells. Biochem Biophys Res Commun. 2019 Nov 15. pii: S0006-291X(19)32179-5. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary and DAPI staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200 overnight at 4°C, DAB staining.