bs-0396R
[Primary Antibody]
VCAM-1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: VCAM-1
Immunogen Range: 640-739/739
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 22329
Swiss Prot: P29533
Source: KLH conjugated synthetic peptide derived from mouse VCAM-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 81
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757.Read more>>
- Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019 Read more>>
- Ding L et al. Saponins of sea cucumber attenuate atherosclerosis in ApoE −/− mice via lipid-lowering and anti-inflammatory properties. Journal of Functional Foods,2018 48, 490–497.Read more>>
- Tianli Yang. et al. Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders:. J Tissue Eng. 2021;()Read more>>
- Chae Eun Kim. et al. Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model. Biomed Pharmacother. 2021 Jul;139:111571Read more>>
- Wei Tang. et al. Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states. Asian J Pharm Sci. 2021 ApRead more>>
- Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
- Xinning Wang. et al. Administration of Donor-Derived Nonexpanded Adipose Stromal Vascular Fraction Attenuates Ischemia-Reperfusion Injury in Donation After Cardiac Death Rat Renal Transplantation. Transpl P. 2021 JulRead more>>
- Xinning Wang. et al. Administration of adipose stromal vascular fraction attenuates acute rejection in donation after circulatory death rat renal transplantation. 2021 Dec 15Read more>>
- Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025Read more>>
- Zhou, Liuhua. et al. Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats. Sci Rep-Uk. 2022 Mar;12(1):1-1Read more>>
- Pu, Luya. et al. Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation. J CELL SCI. 2022 Jul;135(14)Read more>>
- Xin-Sen Chen. et al. Losartan attenuates sepsis-induced cardiomyopathy by regulating macrophage polarization via TLR4-mediated NF-B and MAPK signaling. PHARMACOL RES. 2022 Nov;185:106473Read more>>
- Guangxu Xiao. et al. CXCR1 and its downstream NF-B inflammation signaling pathway as a key target of Guanxinning injection for myocardial ischemia/reperfusion injury.. FRONT IMMUNOL. 2022 Oct;13:1007341-1007341Read more>>
- Yunan Wang. et al. A novel type of mesenchymal stem cells derived from bovine metanephric mesenchyme. TISSUE CELL. 2022 Dec;79:10197Read more>>
- Xing Zeyu. et al. KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65 and IRF3: A Possible Therapeutic Approach for Atherosclerosis. INFLAMMATION. 2023 Jul;:1-18Read more>>
- Chenglin Liang. et al. Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy. ASIAN J PHARM SCI. 2024 Feb;:100888Read more>>
VALIDATION IMAGES
Lane 1: Human VCAM1 protein; Probed with Rabbit Anti-VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Mouse spleen cells (dark blue) were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody, Rabbit Anti-VCAM-1 antibody (bs-0396R, Green) at 1μg /8x10^5 cells was incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then Goat Anti-rabbit IgG/FITC (light blue) antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Isotype control Rabbit IgG (orange) was analyzed under the same conditions.
Lane 1: Human Siha cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with VCAM-1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.