bs-0400R-Cy3 [Conjugated Primary Antibody]
GAD65 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: GAD65

Immunogen Range: 541-567/585

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2572

Swiss Prot: Q05329

Source: KLH conjugated synthetic peptide derived from human GAD65

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wen et al. Striatal and Tegmental Neurons Code Critical Signals for Temporal-Difference Learning of State Value in Domestic Chicks. (2016) Front.Neurosci. 10:476Read more>>
VALIDATION IMAGES

Molt4 cells(black) were fixed with 4% PFA for 10min at room temperature,pemeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GAD65 Polyclonal Antibody(bs-0400R-AF647) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).