bs-0409R [Primary Antibody]
Leptin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Leptin

Immunogen Range: 17-100/167


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 280836

Swiss Prot: P50595

Source: KLH conjugated synthetic peptide derived from bovine Leptin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).Read more>>
  • Zhang, Yun, et al. "Correlation analysis between the expressions of leptin and its receptor (ObR) and clinicopathology in endometrial cancer." Cancer Biomarkers 14.5 (2014): 353-359.Read more>>
  • Li F et al. The Nutritional Cytokine Leptin Promotes NSCLC by Activating the PI3K/AKT and MAPK/ERK Pathways in NSCLC Cells in a Paracrine Manner. Biomed Res Int. 2019 Apr 18;2019:2585743.Read more>>
  • Jisheng Wang. et al. Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats. Med Sci Monitor. 2020; 26: e925579-1Ce925579-11Read more>>
  • Jun Deng. et al. The metabolic hormone leptin promotes the function of T FH cells and supports vaccine responses. Nat Commun. 2021 May;12(1):1-15Read more>>
  • Kimberly A. Clark. et al. Evaluation of the Central Effects of Systemic Lentiviral-Mediated Leptin Delivery in Streptozotocin-Induced Diabetic Rats. Int J Mol Sci. 2021 Jan;22(24):13197Read more>>
  • Michelotti Tainara C.. et al. Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows. FRONT CELL DEV BIOL. 2022 Oct;0:2076Read more>>
  • Pan Jianping. et al. Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway. MOL MED. 2022 Dec;28(1):1-12Read more>>
  • Fangda Fu. et al. AR/PCC herb pair inhibits osteoblast pyroptosis to alleviate diabetes-related osteoporosis by activating Nrf2/Keap1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti-Leptin Polyclonal Antibody (bs-0409R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4°C, DAB staining.


Leptin lysates probed with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.