Host: Rabbit
Target Protein: TIMP-2
Immunogen Range: 131-220/220
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7077
Swiss Prot: P16035
Source: KLH conjugated synthetic peptide derived from human TIMP-2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 24
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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L1 and L2 rat lung lysates probed with Anti TIMP-2 Polyclonal Antibody, Unconjugated (bs-0416R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 24kD. Observed band size: 21kD
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with TIMP2 Polyclonal Antibody, Unconjugated (bs-0416R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.