Host: Rabbit
Target Protein: MOG
Immunogen Range: 35-55/247
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4340
Swiss Prot: Q16653
Source: KLH conjugated synthetic peptide derived from human MOG
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 24
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Pig
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Wei Sun. et al. Ketogenic diet attenuates neuroinflammation and induces conversion of M1 microglia to M2 in an EAE model of multiple sclerosis by regulating the NF-B/NLRP3 pathway and inhibiting HDAC3 and P2X7R activation. FOOD FUNCT. 2023 JulRead more>>
Mouse brain lysates probed with MOG Polyclonal Antibody, unconjugated (bs-0426R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4°C, DAB staining.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Rat Spinal cord tissue lysates probed with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.