bs-0431R
[Primary Antibody]
OPG Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: OPG
Immunogen Range: 221-300/401
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 18383
Swiss Prot: O08712
Source: KLH conjugated synthetic peptide derived from mouse OPG
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF1/TRAIL and protect against apoptosis. TNFSF1/TRAIL binding blocks the inhibition of osteoclastogenesis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Ping. "Efficacy and Safety of Echinacoside in a Rat Osteopenia Model." Evidence-Based Complementary and Alternative Medicine 2013 (2013).Read more>>
- Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9.Read more>>
- Li, Xianxian, et al. "Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." Archives of Oral Biology(2015).Read more>>
- Zhang, Yuanyu, et al. "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-κB ligand and osteoprotegerin in human osteoblasts." Experimental and therapeutic medicine 9.3 (2015): 919-924.Read more>>
- Herath, Steven C., et al. "Stimulation of angiogenesis by cilostazol accelerates fracture healing in mice." Journal of Orthopaedic Research (2015).Read more>>
- Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine (2016).Read more>>
- Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway."Chemico-Biological Interactions (2016).Read more>>
- Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016).Read more>>
- He, Ming, et al. "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." Molecular medicine reports 14.2 (2016): 1054-1060.Read more>>
- Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017). Read more>>
- Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
- Chen, Helin, et al. "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." Archives of Oral Biology (2017).Read more>>
- Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
- Yu X et al. Substance P aggravates periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019.Read more>>
- Yan KX et al. Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019 Dec.Read more>>
- Wei B et al. Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits. J Bone Miner Metab. 2020 Jan 24. Read more>>
- Orth M et al. Effects of locally applied adipose tissue-derived microvascular fragments by thermoresponsive hydrogel on bone healing.Acta Biomater. 2018 Sep 1;77:201-211.Read more>>
- Yang Y et al. Ganoderma lucidum Immune Modulator Protein rLZ-8 Could Prevent and Reverse Bone Loss in Glucocorticoids-Induced Osteoporosis Rat Model. Front Pharmacol. 2020 May 19;11:731.Read more>>
- Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med. 2020 Aug;181:105049.Read more>>
- M M et al. Pantoprazole impairs fracture healing in aged miceSci Rep.2020 Dec 23;10(1):22376.Read more>>
- Daisuke Nishida. et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep-Uk. 2021 Feb;11(1):1-12Read more>>
- Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
- Wang Tong. et al. Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8168657Read more>>
- Yutao Cui. et al. Functionalized anti-osteoporosis drug delivery system enhances osseointegration of an inorganicCorganic bioactive interface in osteoporotic microenvironment. Mater Design. 2021 Aug;206:109753Read more>>
- Shan Shan Qi. et al. Lycopene ameliorates diabetic osteoporosis via anti-inflammatory, anti-oxidation, and increasing Osteoprotegerin/RANKL expression ratio. J Funct Foods. 2021 Aug;83:104539Read more>>
- aixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-42Read more>>
- Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094Read more>>
- Hiroki Ehara. et al. Role of tissue factor in delayed bone repair induced by diabetic state in mice. Plos One. 2021 Dec;16(12):e0260754Read more>>
- Ruijun Su. et al. Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE-/- mice. Ecotox Environ Safe. 2022 Apr;234:11341Read more>>
- Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
- Wang, YunFei. et al. Inactivation of Ihh in Sp7-Expressing Cells Inhibits Osteoblast Proliferation, Differentiation, and Bone Formation, Resulting in a Dwarfism Phenotype with Severe Skeletal Dysplasia in Mice. CALCIFIED TISSUE INT. 2022 Jun;:1-16Read more>>
- Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913Read more>>
- Yang Man. et al. Therapeutic Potential of Liraglutide for DiabetesPeriodontitis Comorbidity: Killing Two Birds with One Stone. J DIABETES RES. 2022;2022:8260111Read more>>
- Xue Jiang. et al. Regulation of Wnt Signaling Pathway by Costic Acid Derivative, An Efficient Strategy for Treatment of Glucocorticoid-Induced Osteoporosis in Rat Model. CHEMISTRYSELECT. 2023 Apr;8(13):e202204912Read more>>
- Cheng Lanlan. et al. Liraglutide attenuates palmitate-induced apoptosis via PKA/-catenin/Bcl-2/Bax pathway in MC3T3-E1 cells. N-S ARCH PHARMACOL. 2023 Jul;:1-13Read more>>
- ohui Liu. et al. Near-Infrared Light-Controlled MicroRNA-21-Loaded Upconversion Nanoparticles to Promote Bone Formation in the Midpalatal Suture. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Hongxing Zheng. et al. Chromium Picolinate Regulates Bone Metabolism and Prevents Bone Loss in Diabetic Rats. MOLECULES. 2024 Jan;29(5):924Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-Osteoprotegerin Polyclonal Antibody (bs-0431R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse heart lysates; Lane 2: Mouse kidney lysates; Lane 3: Mouse liver lysates probed with OPG Polyclonal Antibody (bs-0431R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Lane 1: Human Raji cell lysates; Lane 2: Human U251 cell lysates probed with OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.