bs-0441R [Primary Antibody]
ChRM2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ChRM2

Immunogen Range: 168-250/466


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1129

Swiss Prot: P08172

Source: KLH conjugated synthetic peptide derived from human ChRM2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.Read more>>
  • Zeng H et al. Transcriptomic analysis of α-synuclein knockdown after T3 spinal cord injury in rats. BMC Genomics. 2019 Nov 14;20(1):851. Read more>>
  • Tong L et al. Effects of topical pilocarpine on ocular growth and refractive development in rabbits. Eur J Ophthalmol. 2020 Jun 11;1120672120934962.Read more>>
  • Liyang Tong. et al. Topical bendazol inhibits experimental myopia progression and decreases the ocular accumulation of HIF\1 protein in young rabbits. Ophthal Physl Opt. 2020 Sep;40(5):567-576Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti ChRM2 Polyclonal Antibody, Unconjugated (bs-0441R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ChRM2 Polyclonal Antibody, Unconjugated (bs-0441R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ChRM2) Polyclonal Antibody, Unconjugated (bs-0441R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Heart tissue lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0441R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.