Host: Rabbit
Target Protein: ACTH 1-39
Immunogen Range: 1-39/39
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5443
Swiss Prot: P01189
Source: KLH conjugated synthetic peptide derived from human ACTH
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 4.5
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Cow
Goat
For research use only. Not intended for diagnostic or therapeutic use.
- Horiguchi K et al. CX3CL1/CX3CR1-signalling in the CD9/S100β/SOX2-positive adult pituitary stem/progenitor cells modulates differentiation into endothelial cells. Histochem Cell Biol. 2020 Mar 9. Read more>>
- Hui Ye. et al. Triggering receptor expressed on myeloid Cells-2 (TREM2) inhibits steroidogenesis in adrenocortical cell by macrophage-derived exosomes in lipopolysaccharide-induced septic shock. Mol Cell Endocrinol. 2021 Apr;525:111178Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ACTH (1-39) Polyclonal Antibody, Unconjugated (bs-0443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ACTH (1-39) Polyclonal Antibody, Unconjugated(bs-0443R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human pituitary tumour; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ACTH (1-39) Polyclonal Antibody (bs-0443R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.