Host: Rabbit
Target Protein: NIS
Immunogen Range: 525-618/618
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 114479
Swiss Prot: Q99PN0
Source: KLH conjugated synthetic peptide derived from mouse NIS
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 68
Cross Reactive Species:
Human
Rat
Pig
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
- Guo, Hongwei, et al. "Molecular mechanisms of human thyrocyte dysfunction induced by low concentrations of polychlorinated biphenyl 118 through the Akt/FoxO3a/NIS pathway." Journal of Applied Toxicology (2015).Read more>>
- Yang H, Chen H, Guo H, Li W, Tang J, et al. (2015) Molecular Mechanisms of 2, 3′, 4, 4′, 5-Pentachlorobiphenyl-Induced Thyroid Dysfunction in FRTL-5 Cells. PLoS ONE 10(3): e0120133.Read more>>
- Yao, Chen, et al. "Effect of sodium/iodide symporter (NIS)-mediated radioiodine therapy on estrogen receptor-negative breast cancer." Oncology Reports.Read more>>
- Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80Read more>>
- Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats. Ecotoxicol Environ Saf 2021 Jan 15;208:111720.Read more>>
- Shasha Hou. et al. Downregulation of miR-146b-3p Inhibits Proliferation and Migration and Modulates the Expression and Location of Sodium/Iodide Symporter in Dedifferentiated Thyroid Cancer by Potentially Targeting MUC20. Front Oncol. 2020; 10: 566365Read more>>
- Le Xiong. et al. Biological implications of PTEN upregulation and altered sodium/iodide symporter intracellular distribution in resveratrol-suppressed anaplastic thyroid cancer cells. J Cancer. 2020; 11(23): 6883C6891Read more>>
- Siying Tang. et al. GLIS3 mediated by the Rap1/PI3K/AKT signal pathway facilitates real-ambient PM2.5 exposure disturbed thyroid hormone homeostasis regulation. Ecotox Environ Safe. 2022 Mar;232:113248Read more>>
- Niu Mengda. et al. Evaluation of [18F]tetrafluoroborate as a Potential PET Imaging Agent in a Sodium Iodide Symporter-Transfected Cell Line A549 and Endogenous NIS-Expressing Cell Lines MKN45 and K1. Mol Imaging. 2022;2022:267926Read more>>
- Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13Read more>>
- Jiang B.. et al. Primary human thyrocytes maintained the function of thyroid hormone production and secretion in vitro. J ENDOCRINOL INVEST. 2023 May;:1-12Read more>>
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat thyroid gland labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 65kD. Observed band size:65kD.
Formalin-fixed and paraffin-embedded human thyroid labeled with Rabbit Anti-NIS Polyclonal Antibody, Unconjugated(bs-448R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Lane 1: Mouse Stomach tissue lysates; Lane 2: Rat Stomach tissue lysates probed with NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.