bs-0466R [Primary Antibody]
beta casein Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: beta casein

Immunogen Range: 151-222/222


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 443391

Swiss Prot: P11839

Source: KLH conjugated synthetic peptide derived from sheep Beta-casein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Important role in determination of the surface properties of the casein micelles.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Bovine
Sheep
Goat
Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang W et al. The inhibitory effect of melatonin on mammary function of lactating dairy goats.(2018) Biol Reprod. Oct 22.Read more>>
  • Li P et al. CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. J Agric Food Chem. 2019 Sep 18;67(37):10513-10520. Read more>>
  • Jinyu Zhou et al. Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows. J Dairy Sci. 2020 Jul;103(7):6627-6634.Read more>>
  • Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res. 2020 Aug 7;19(8):3211-3219.Read more>>
  • Ludmila K et al. Development of Immunohistochemical Methods for Casein Detection in Meat ProductsFoods.2020 Dec 24;10(1):28.Read more>>
  • Z.Y. Hao. et al. Deep small RNA-Seq reveals microRNAs expression profiles in lactating mammary gland of 2 sheep breeds with different milk performance. Domest Anim Endocrin. 2021 Jan;74:106561Read more>>
  • Zhiyun Hao. et al. MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells. Food Funct. 2021 AugRead more>>
  • Hao Qi. et al. Methionine and leucine induce ARID1A degradation to promote mTOR expression and milk synthesis in mammary epithelial cells. J Nutr Biochem. 2021 Nov;:108924Read more>>
  • Xinling Wang. et al. Enhancement of BCAT2-Mediated Valine Catabolism Stimulates -Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Xinling Wang. et al. Plasma Proteomic Profiling Reveals the Regulatory Factors of Milk Protein Synthesis in Holstein Cows. BIOLOGY-BASEL. 2022 Aug;11(8):1239Read more>>
  • Gang Lin. et al. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J NUTR BIOCHEM. 2023 Jan;:109274Read more>>
  • Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708Read more>>
  • Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2CSTAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451Read more>>
VALIDATION IMAGES

Lane 1: Milk (Goat) Lysate at 2 ug Lane 2: Milk (Cow) Lysate at 30 ug Lane 3: Milk (Cow) Lysate at 3 ug Lane 4: Milk (Human) Lysate at 30 ug Primary: Anti-Beta-casein (bs-0466R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution