bs-0468R-A350
[Conjugated Primary Antibody]
CD31 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
DATASHEET
Host: Rabbit
Target Protein: CD31
Immunogen Range: 601-680/738
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5175
Swiss Prot: P16284
Source: KLH conjugated synthetic peptide derived from mouse CD31
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-69 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Conjugation: ALEXA FLUOR® 350
Excitation/ Emission: 346nm/442nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 78
Cross Reactive Species:
Human
Mouse
Rat
Dog
Pig
Rabbit
Predicted Cross Reactive Species: Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
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- Sheng Cao. et al. Ultrasound-targeted microbubble destruction-mediated Ang1 gene transfection improves left ventricular structural and sympathetic nerve remodeling in canines with myocardial infarction. Ann Transl Med. 2021 Feb; 9(3): 221Read more>>
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- Norihiro Muroi. et al. Effects of long-term plate fixation with different fixation modes on the radial cortical bone in dogs. Plos One. 2021 Feb;16(2):e024741Read more>>
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- Yamei Zhou. et al. CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization. Invest Ophth Vis Sci. 2021 Mar;62(3):37-37Read more>>
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- Lin Chih-Hsun. et al. Frozen Fat Grafts Maintain Vascular Endothelial Growth Factor Expression and Mediate Angiogenesis During Adipose-Derived Stem Cell Enrichment for Soft Tissue Augmentation. 2022 Mar 01Read more>>
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- Hongqi Meng. et al. Hydrogels Containing Chitosan-Modified Gold Nanoparticles Show Significant Efficacy in Healing Diabetic Wounds Infected with Antibiotic-Resistant Bacteria. INT J NANOMED. 2024 Feb 18Read more>>
VALIDATION IMAGES