Host: Rabbit
Target Protein: Adiponectin
Immunogen Range: 151-244/244
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9370
Swiss Prot: Q15848
Source: KLH conjugated synthetic peptide derived from human Adiponectin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Pig
Predicted Cross Reactive Species:
Dog
Cow
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Cao, Zhongzan, et al. "Molecular cloning and expression analysis of adiponectin and its receptors (AdipoR1 and AdipoR2) in the hypothalamus of the Huoyan goose during different stages of the egg-laying cycle."Reproductive Biology and Endocrinology 13.1 (2015): 87.Read more>>
- Du et al. MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat. (2018) Int.J.Mol.Sci. 19 Read more>>
- Wang F et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24. Read more>>
- Zhang K et al. Insulin‑like growth factor 2 promotes the adipogenesis of hemangioma‑derived stem cells. Exp Ther Med. 2019 Mar;17(3):1663-1669. Read more>>
- Khan R et al. Bta‐miR‐149‐5p inhibits proliferation and differentiation of bovine adipocytes through targeting CRTCs at both transcriptional and posttranscriptional levels. J Cell Physiol. 2020 Jan 31.Read more>>
- Haodong Liu. et al. miR\340\5p inhibits sheep adipocyte differentiation by targeting ATF7. Anim Sci J. 2020 Jan;91(1):e13462Read more>>
- Pengyu Hong. et al. Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regenerationan in vitro and in vivo study. Stem Cell Res Ther. 2021 Dec;12(1):1-13Read more>>
- Xiaoyu Wang. et al. RNA-Seq and lipidomics reveal different adipogenic processes between bovine perirenal and intramuscular adipocytes. ADIPOCYTE. 2022 Aug 08Read more>>
- Chungho Lee. et al. Disturbance of lipid metabolism in germ-free mice transplanted with gut microbiota of DSS-induced colitis mice. PLOS ONE. 2023 Feb;18(2):e028085Read more>>
- Chen Manyun. et al. Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin. NPJ BIOFILMS MICROBI. 2023 Jul;9(1):1-15Read more>>
- Shun Watanabe. et al. Genistein enhances NAD+ biosynthesis by upregulating nicotinamide phosphoribosyltransferase in adipocytes. J NUTR BIOCHEM. 2023 Aug;:109433Read more>>
- W.J. Tu. et al. Osteocalcin activates lipophagy via the ADPN-AMPK/PPAR-mTOR signaling pathway in chicken embryonic hepatocyte. POULTRY SCIENCE. 2023 Nov;:103293Read more>>
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 25kD. Observed band size:25kD.
Mouse spleen lysate probed (bs-0471R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.\n
HepG2 lysate probed with Anti-Adiponectin Polyclonal Antibody (bs-0471R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.