bs-0472R [Primary Antibody]
GLUT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GLUT1

Immunogen Range: 191-270/492


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6513

Source: KLH conjugated synthetic peptide derived from human GLUT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yun S et al. Effects of lead exposure on brain glucose metabolism and insulin signaling pathway in the hippocampus of rats. Toxicol Lett. 2019 Aug;310:23-30.Read more>>
  • Hu C et al. Placentae for Low Birth Weight Piglets Are Vulnerable to Oxidative Stress, Mitochondrial Dysfunction, and Impaired Angiogenesis. Oxid Med Cell Longev. 2020 May 25;2020:8715412.Read more>>
  • Zhao, Ying. et al. Apigenin increases radiosensitivity of glioma stem cells by attenuating HIF-1-mediated glycolysis. Med Oncol. 2021 Nov;38(11):1-1Read more>>
  • Chen Mengyuan. et al. Celastrol Protects against Cerebral Ischemia/Reperfusion Injury in Mice by Inhibiting Glycolysis through Targeting HIF-1_/PDK1 Axis. Oxid Med Cell Longev. 2022;2022:7420507Read more>>
  • Changhao Jia. et al. Apigenin sensitizes radiotherapy of mouse subcutaneous glioma through attenuations of cell stemness and DNA damage repair by inhibiting NF-_B/HIF-1_-mediated glycolysis. J NUTR BIOCHEM. 2022 May;:109038Read more>>
  • Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176Read more>>
  • Huang, Hui. et al. Osthole increases the radiosensitivity of hepatoma cells by inhibiting GSK-3/AMPK/mTOR pathway-controlled glycolysis. N-S ARCH PHARMACOL. 2022 Nov;:1-1Read more>>
  • Karayakali, Melike. et al. Crocin treatment exerts anti-inflammatory and anti-oxidative effects in liver tissue damage of pinealectomized diabetic rats. ENVIRON SCI POLLUT R. 2023 Feb;:1-15Read more>>
  • Kexin Wang. et al. OBHS Drives Abnormal Glycometabolis Reprogramming via GLUT1 in Breast Cancer. INT J MOL SCI. 2023 Jan;24(8):7136Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLUT1 Polyclonal Antibody (bs-0472R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (bs-0472R-PE)at 1:50.


Lane 1: Mouse Heart lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Muscle lysates; Lane 4: Rat Cerebrum lysates probed with GLUT1 Polyclonal Antibody, Unconjugated (bs-0472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.