bs-0480R-A488 [Conjugated Primary Antibody]
IFN gamma Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IFN gamma

Specificity: This antibody has been shown to work in frozen tissue section IHC as well, but is not guaranteed for this application.

Immunogen Range: 75-155/155


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 15978

Swiss Prot: P01580

Source: KLH conjugated synthetic peptide derived from mouse IFN gamma

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Huibin, et al. "Upregulation of thyroid transcription factor-1 and human leukocyte antigen class I in Hashimoto's disease providing a clinical evidence for possible triggering autoimmune reaction." European Journal of Endocrinology 164.5 (2011): 795-800.Read more>>
  • Lu, Na-na, et al. "Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1." Evidence-Based Complementary and Alternative Medicine 2014 (2014).Read more>>
  • Ishida, Waka, et al. "Inhibition of very late antigen-4 and leukocyte function-associated antigen-1 in experimental autoimmune uveoretinitis." Clinical Immunology (2014).Read more>>
  • Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
  • Zhou, Xue, et al. "Sulindac has strong antifibrotic effects by suppressing STAT3‐related miR‐21." Journal of Cellular and Molecular Medicine (2015).Read more>>
  • Gong, Yu-Bo, et al. "Effects of Foxp3 gene modified dendritic cells on mouse corneal allograft rejection." International Journal of Clinical and Experimental Medicine 8.3 (2015): 3965-3973.Read more>>
  • Li, Peiqi, et al. "The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering."International Journal of Molecular Sciences 16.10 (2015): 25678-25690.Read more>>
  • Venkatasubramanian, Sambasivan, et al. "Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection." European Journal of Immunology (2015).Read more>>
  • Makar, Tapas K., et al. "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." Journal of Neuroinflammation 12.1 (2015): 1-13.Read more>>
  • Makar, Tapas K., et al. "TrkB agonist, 7, 8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis."Journal of Neuroimmunology (2016).Read more>>
  • Li, Peiqi, et al. "Interferon-γ enhances the efficacy of autogenous bone grafts by inhibiting postoperative bone resorption in rat calvarial defects." Journal of Prosthodontic Research (2016).Read more>>
  • Kim, Dong-Kee, et al. "Otitis Media with Effusion in an Allergic Animal Model: a Functional and Morphological Study." International Journal of Pediatric Otorhinolaryngology (2016).Read more>>
  • Cao, Yu, et al. "l-arginine and docetaxel synergistically enhance anti-tumor immunity by modifying the immune status of tumor-bearing mice."International Immunopharmacology 35 (2016): 7-14.Read more>>
  • Jie, Guo, et al. "Expression of interleukin-17 in autoimmune dacryoadenitis in MRL/lpr mice." Current eye research 35.10 (2010): 865-871.Read more>>
  • Zhang, Qian-Qian, et al. "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." Scientific reports 5 (2015).Read more>>
  • Liang, Zhuoping, et al. "Role of dendritic cells in consistency between upper and lower airway inflammatory responses: animal experimental studies." Int J Clin Exp Pathol 9.10 (2016): 10093-10104.Read more>>
  • Chuang, Meng-Hsien, et al. "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." BMC Complementary and Alternative Medicine 17.1 (2017): 210.Read more>>
  • Tuorkey, Muobarak J. "Effects of Moringa oleifera aqueous leaf extract in alloxan induced diabetic mice." Interventional Medicine and Applied Science 8.3 (2016): 109-117.Read more>>
  • Wang, Xinhong, et al. "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." Cytokine (2017).Read more>>
  • Hayashi et al. Uncarboxylated Osteocalcin Induces Antitumor Immunity against Mouse Melanoma Cell Growth. (2017) J.Cancer. 8:2478-2486Read more>>
  • Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
  • Hou DD et al. Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis. Int Immunopharmacol. 2019 Jun 7;74:105676.Read more>>
  • Yang R et al. Cordycepin Attenuates IFN-γ-Induced Macrophage IP-10 and Mig Expressions by Inhibiting STAT1 Activity in CFA-Induced Inflammation Mice Model. Inflammation. 2019 Dec 23.Read more>>
  • Yamada H et al. LATE-ONSET ALZHEIMER'S DISEASE ANIMAL MODEL AND USES THEREOF. US Patent20190387723Read more>>
  • Li G et al. Effects of combined treatment with PD‑L1 Ig and CD40L mAb on immune tolerance in the CBA/J x DBA/2 mouse model. Molecular Medicine Reports, 2020, 21, 1789-1798.Read more>>
  • Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
  • Fei W et al. The effects of aqueous extract of Maca on energy metabolism and immunoregulation. Eur J Med Res. 2020 Jun 29;25(1):24.Read more>>
  • Qingqing Chen. et al. Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity. J Ethnopharmacol. 2021 Mar;267:113542Read more>>
  • Xin Hu. et al. The role of CXCR3 and its ligands expression in Brucellar spondylitis. Bmc Immunol. 2020 Dec;21(1):1-8Read more>>
  • Chinthalapally V. Rao. et al. GSK3\ARC/Arg3.1 and GSK3\Wnt signaling axes trigger amyloid\ accumulation and neuroinflammation in middle\aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221Read more>>
  • Tingting Guo. et al. Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. Carbohyd Polym. 2020 Dec;250:116904Read more>>
  • Jun Tan. et al. Small molecules targeting RORt inhibit autoimmune disease by suppressing Th17 cell differentiation. Cell Death Dis. 2020 Aug;11(8):1-14Read more>>
  • Jia-xiang Zhang. et al. Kupffer cell depletion attenuates IL-6/STAT3 mediates hepatocyte apoptosis in immunological liver injury of trichloroethylene sensitized mice. Int Immunopharmacol. 2020 Nov;88:106897Read more>>
  • Yu Shan. et al. miR-98-5p protects against cerebral ischemia/reperfusion injury through anti-apoptosis and anti-oxidative stress in mice. J Biochem. 2021 Mar;169(2):195-206Read more>>
  • Gaolong Zhong. et al. Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks. Sci Total Environ. 2021 Sep;788:14778Read more>>
  • Shuling Zhang. et al. Methionine enkephalin (MENK) suppresses lung cancer by regulating the Bcl-2/Bax/caspase-3 signaling pathway and enhancing natural killer cell-driven tumor immunity. Int Immunopharmacol. 2021 Sep;98:107837Read more>>
  • Shuling Zhang. et al. A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment. Int Immunopharmacol. 2021 Oct;99:107999Read more>>
  • Ying Xue. et al. Application of TGF?1, TIMP?1 and TIMP?2 small interfering RNAs can alleviate CCl<sub>4</sub>?induced hepatic fibrosis in rats by rebalancing Th1/Th2 cytokines. Exp Ther Med. 2021 Sep;22(3):1-7Read more>>
  • Li, Jing. et al. Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration. Cell Commun Signal. 2021 Dec;19(1):1-2Read more>>
  • Qingqing Liu. et al. Probiotics alleviate autoimmune hepatitis in mice through modulation of gut microbiota and intestinal permeability. J Nutr Biochem. 2021 Sep;:108863Read more>>
  • Wen Hua DUAN. et al. 2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages. Biomed Environ Sci. 2021 Sep;34:693Read more>>
  • Chi-Wei Huang. et al. Creation of a rat lymphedema model using extensive lymph node dissection and circumferential soft tissue resection: Is this a reliable model?. Microsurg. 2021 Nov;41(8):762-771Read more>>
  • Yongbo Kang. et al. Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability. Pharmacol Res. 2021 Dec;:10602Read more>>
  • An Wang. et al. Yiqi Jiedu herbal decoction attenuates the 2?Gy 60Co ray induced spleen injury by inhibiting apoptosis and modulating the immune balance. J Ethnopharmacol. 2022 Mar;286:114925Read more>>
  • Meng-Meng ZHANG. et al. Protective effect of Pai-Nong-San against AOM/DSS-induced CAC in mice through inhibiting the Wnt signaling pathway. Chin J Nat Medicines. 2021 Dec;19:912Read more>>
  • Minghui Li. et al. Rho Kinase regulates neutrophil NET formation that is involved in UVB-induced skin inflammation. Theranostics. 2022; 12(5): 21332149Read more>>
  • I.B. Reis. et al. OncoTherad (MRB-CFI-1) nano-immunotherapy reduced tumoral progression in non-muscle invasive bladder cancer through activation of Toll-like signaling pathway. Tissue Cell. 2022 Jun;76:101762Read more>>
  • Maoyu Ye. et al. Long-Term Exposure to Sulfur Dioxide Before Sensitization Decreased the Production of Specific IgE in HDM-Sensitized Allergic Rhinitis Mice. J INFLAMM RES. 2022; 15: 2477249Read more>>
  • Dian-Dong Hou. et al. Therapeutic effects of myricetin on atopic dermatitis in vivo and in vitro. PHYTOMEDICINE. 2022 Jul;102:1542Read more>>
  • Kai Ye. et al. An armed oncolytic virus enhances the efficacy of tumor-infiltrating lymphocyte therapy by converting tumors to artificial antigen presenting cells in situ. MOL THER. 2022 JuRead more>>
VALIDATION IMAGES