bs-0486R [Primary Antibody]
Integrin beta 1 Polyclonal Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: Integrin beta 1

Immunogen Range: 25-100/798

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3688

Swiss Prot: P05556

Source: KLH conjugated synthetic peptide derived from human Integrin beta 1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)

Predicted Molecular Weight: 88

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

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Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Integrin beta 1/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

A549 cells(black) were fixed with 4% PFA for 10min at room temperature,pemeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin beta 1 Polyclonal Antibody(bs-0486R-AF488) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Lane 1: Human A431 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Rat Ear lysates; Lane 4: Mouse Ear lysates; Lane 5: Mouse Urinary bladder lysates probed with Integrin beta 1 Polyclonal Antibody, Unconjugated (bs-0486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Integrin beta 1) polyclonal Antibody, Unconjugated (bs-0486R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.