bs-0486R-A488 [Conjugated Primary Antibody]
Integrin beta 1 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Integrin beta 1

Immunogen Range: 25-100/798

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3688

Swiss Prot: P05556

Source: KLH conjugated synthetic peptide derived from human Integrin beta 1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at 4°C for 12 months.

Background:

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 88

Cross Reactive Species: Human
Mouse
Rat
Sheep
Rabbit
Others

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Xiaodong, et al. "The effect of porcine ADM to improve the burn wound healing." Int J Clin Exp Pathol 6.11 (2013): 2280-2291.Read more>>
  • Lei, Mingxing, et al. "Modulating hair follicle size with Wnt10b/DKK1 during hair regeneration." Experimental Dermatology (2014).Read more>>
  • Jiang, Xiaorong, et al. "The role of CD29-ILK-Akt signaling-mediated epithelial–mesenchymal transition of liver epithelial cells and chemoresistance and radioresistance in hepatocellular carcinoma cells." Medical Oncology 32.5 (2015): 1-8.Read more>>
  • Deng, Wei, et al. "Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis." International Journal of Clinical and Experimental Pathology 8.9 (2015): 10555-10564.Read more>>
  • Qin, Jie, et al. "Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway." International Journal of Molecular Medicine.Read more>>
  • Wang, Yan, et al. "Effects of panax notoginseng saponins on the osteogenic differentiation of rabbit bone mesenchymal stem cells through TGF-β1 signaling pathway." BMC Complementary and Alternative Medicine 16.1 (2016): 319.Read more>>
  • Oralova, V., et al. "Osteogenic Potential of the Transcription Factor c-MYB." Calcified Tissue International: 1-12.Read more>>
  • Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
  • Fu, Min, et al. "Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice." Nutrients 8.5 (2016): 286.Read more>>
  • Zhao, Xue, et al. "Knockdown of Snail inhibits epithelial-mesenchymal transition of human laryngeal squamous cell carcinoma Hep-2 cells through VDR signaling pathway." Biochemistry and Cell Biology ja (2017).Read more>>
  • Ji, Kaiyuan, et al. "MicroRNA 143‐5p regulates alpaca melanocyte migration, proliferation, and melanogenesis." Experimental dermatology (2017).Read more>>
  • Wang et al. Loss of calponin h1 confers anoikis resistance and tumor progression in the development of high-grade serous carcinoma originating from the fallopian tube epithelium. (2017) Oncotarget. 8:61133-61145Read more>>
  • Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
  • Lu T et al. In vitro culture and biological properties of broiler adipose‑derived stem cells.Exp Ther Med. 2018 Sep;16(3):2399-2407.Read more>>
  • Gao Y et al. Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Exp Ther Med. 2020 Mar;19(3):2202-2210.Read more>>
  • Heng Chen. et al. Podosome formation in the murine palatal mucosae: Its proteolytic role in rete peg formation. Ann Anat. 2021 May;235:151703Read more>>
  • Ziwei Luo. et al. miR\203a\3p promotes loureirin A\induced hair follicle stem cells differentiation by targeting Smad1. Anat Rec. 2021 Mar;304(3):531-54Read more>>
  • Laijun Liu. et al. Hierarchical Nanostructured Electrospun Membrane with Periosteum-Mimic Microenvironment for Enhanced Bone Regeneration. 2021 Aug 05Read more>>
  • Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359Read more>>
  • Shuying Hu. et al. Superparamagnetic coreCshell electrospun scaffolds with sustained release of IONPs facilitating in vitro and in vivo bone regeneration. J Mater Chem B. 2021 AugRead more>>
  • Ge Liu. et al. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I. J Biol Chem. 2021 Aug;:101133Read more>>
  • Xingyu Shen. et al. Extracellular Calcium Ion Concentration Regulates Chondrocyte Elastic Modulus and Adhesion Behavior. Int J Mol Sci. 2021 Jan;22(18):10034Read more>>
VALIDATION IMAGES

A549 cells(black) were fixed with 4% PFA for 10min at room temperature,pemeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin beta 1 Polyclonal Antibody(bs-0486R-AF488) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).