bs-0489R-A647 [Conjugated Primary Antibody]
BrdU Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: BrdU

Clonality: Polyclonal

Isotype: IgG

Source: BrdU conjugated to KLH

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at 4°C for 12 months.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: IF(IHC-P)(1:50-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

  • Zhang, Rui, et al. "Neuroprotective Effects of Sulforaphane on Cholinergic Neurons in Mice with Alzheimer’s Disease-Like Lesions." International Journal of Molecular Sciences 15.8 (2014): 14396-14410.Read more>>
  • Giuliani, Daniela, et al. "NDP-α-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC 4 receptors." Molecular and Cellular Neuroscience (2015).Read more>>
  • Chen et al. Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro. (2014) Cell.Physiol.Bioche. 34:1109-24Read more>>
  • Zhang et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. (2015) World.J.Gastroenterol. 21:4195-209Read more>>

Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10μM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.