Host: Rabbit
Target Protein: Dnmt3a
Immunogen Range: 26-100/912
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1788
Swiss Prot: Q9Y6K1
Source: KLH conjugated synthetic peptide derived from human Dnmt3a
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 100
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Zhao, Qian, et al. "Prenatal Cocaine Exposure Impairs Cognitive Function of Progeny Via Insulin Growth Factor II Epigenetic Regulation." Neurobiology of Disease (2015).Read more>>
- Gökmen, Esra, and Kemal Ergin. "DNA Methyltransferase Expression and Proliferation Status of Metastatic Breast Cancer Cell Line After Prolonged and Repeated Rapamycin and Melatonin Application." Meandros Medical Journal.Read more>>
- Wu, Yuting, et al. "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." Toxicology and Applied Pharmacology 315 (2017): 35-49.Read more>>
- Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
- Jiachen Wang. et al. Upregulation of miR-137 Expression Suppresses Tumor Growth and Progression via Interacting with DNMT3a Through Inhibiting the PTEN/Akt Signaling in HCC. Oncotargets Ther. 2021; 14: 165C176Read more>>
- Ai Wei. et al. Inhibition of DNA methylation derepresses PPAR and attenuates pulmonary fibrosis. 2021 Aug 11Read more>>
- Dr. xingren Chen. et al. Reversal of epigenetic PPAR_ suppression by diacerein alleviates oxidative stress and osteoarthritis in mice. 2022 Feb 24Read more>>
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-Dnmt3a Polyclonal Antibody, Unconjugated (bs-0497R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Dnmt3a Polyclonal Antibody, Unconjugated (bs-0497R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. SH-SY5Y cells probed with Dnmt3a Polyclonal Antibody (bs-0497R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
U-87MG cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Dnmt3a Antibody(bs-R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).