bs-0511R [Primary Antibody]
FADD Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FADD

Immunogen Range: 1-80/205


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14082

Swiss Prot: Q61160

Source: KLH conjugated synthetic peptide derived from mouse FADD

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (By similarity). Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse
Rat
Pig
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Soni, Hitesh, and Adebowale Adebiyi. "Early septic insult in neonatal pigs increases serum and urinary soluble Fas ligand and decreases kidney function without inducing significant renal apoptosis." Renal Failure (2016): 1-12.Read more>>
  • Ji and Ji Laminarin-induced apoptosis in human colon cancer LoVo cells. (2014) Oncol.Lett. 7:1728-1732Read more>>
  • Yang et al. Sialic acid rescues repurified lipopolysaccharide-induced acute renal failure via inhibiting TLR4/PKC/gp91-mediated endoplasmic reticulum stress, apoptosis, autophagy, and pyroptosis signaling. (2014) Toxicol.Sci. 141:155-65Read more>>
  • Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163.Read more>>
  • Luan Jing. et al. Inhibition of CHRM3 Alleviates Necrosis Via the MAPK-p38/miR-31-5p/RIP3 Axis in l-ArginineCInduced Severe Acute Pancreatitis. Pancreas. 2020 Nov;49(10):1335-1341Read more>>
VALIDATION IMAGES

L1 mouse liver lysates, L2 mouse brain lysates probed with Anti FADD Polyclonal Antibody, Unconjugated (bs-0511R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 23kD


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FADD Polyclonal Antibody (bs-0511R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FADD Polyclonal Antibody (bs-0511R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.