bs-0519R
[Primary Antibody]
ATF3 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ATF3
Immunogen Range: 51-150/181
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 467
Swiss Prot: P18847
Source: KLH conjugated synthetic peptide derived from human ATF3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(WB=1:500-2000)
ELISA(ELISA=1:5000-10000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Predicted Molecular Weight: 21
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hu, Jianran, Yu Li, and Ping Li. "MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1." PLOS ONE 8.6 (2013): e68291.Read more>>
- Feng, J. et al. "Upregulation of ATF-3 is correlated with prognosis and proliferation of laryngeal cancer by regulating Cyclin D1 expression" International Journal of Clinical and Experimental Pathology 2013;6(10):2064-2070Read more>>
- Aguirre, Alina, et al. "Defective autophagy impairs ATF3 activity and worsens lung injury during endotoxemia." Journal of Molecular Medicine (2014): 1-12.Read more>>
- Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.Read more>>
- Smith, Carolyne K. The Interplay of Lipoproteins and Innate Immune Responses in Systemic Lupus Erythematosus and its Role in Premature Cardiovascular Disease. Diss. University of Michigan, 2015.Read more>>
- Smith, Carolyne K., et al. "Lupus high-density lipoprotein induces proinflammatory responses in macrophages by binding lectin-like oxidised low-density lipoprotein receptor 1 and failing to promote activating transcription factor 3 activity." Annals of the Rheumatic Diseases (2016): annrheumdis-2016.Read more>>
- Xu HY et al. Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3. Biochemistry (Mosc). 2020 Jul;85(7):749-757.Read more>>
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Magadmi Rania M.. et al. The Neuroprotective Effect of Carvedilol on Diabetic Neuropathy: An In Vitro Study. J Diabetes Res. 2021;2021:6927025Read more>>
- Rongxue Wei. et al. Study on the Effect of Different Types of Sugar on Lipid Deposition in Goose Fatty Liver. Poultry Sci. 2022 Jan;:101729Read more>>
- Ramona Palombo. et al. Inhibition of the PI3K/AKT/mTOR signaling promotes an M1 macrophage switch by repressing the ATF3-CXCL8 axis in Ewing sarcoma. CANCER LETT. 2023 Feb;555:216042Read more>>
- Jinxin Ma. et al. Bioinformatics and Integrative Experimental Method to Identifying and Validating Co-Expressed Ferroptosis-Related Genes in OA Articular Cartilage and Synovium. J INFLAMM RES. 2024 Feb 11Read more>>
- Xiangyu Li. et al. Exploring the mechanism of Nav1.3 in the ION-CCI rat model based on the TLR4/TRAF6/NF-B pathway. NEUROSCI LETT. 2024 May;832:137806Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-ATF3 Polyclonal Antibody, Unconjugated (bs-0519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-ATF3 Polyclonal Antibody, Unconjugated (bs-0519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse lung lysate Lane 2: mouse brain lysate probed with Rabbit Anti-ATF3 Polyclonal Antibody, Unconjugated (bs-0519R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Human 293T cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human HepG2 cell lysates probed with ATF1 Polyclonal Antibody, Unconjugated (bs-0519R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
RAW264.7 (M) cells were treated with or without LPS (1ug/ml) for 2 h, 25 μg total protein per lane of cell lysates (see on figure) probed with ATF3 polyclonal antibody, unconjugated (bs-0519R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATF3 Polyclonal Antibody, Unconjugated (bs-0519R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.