Host: Rabbit
Target Protein: PKA alpha + beta
Immunogen Range: 17-100/351
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 282323
Swiss Prot: P05131
Source: KLH conjugated synthetic peptide derived from bovine PKA
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 40
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Yang Liuet al. Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway. Exp Ther Med. 2020 Jul;20(1):495-499.Read more>>
- Yongxiang Li. et al. Food reward depends on TLR4 activation in dopaminergic neurons. Pharmacol Res. 2021 Jul;169:105659Read more>>
- Tong Hongxuan. et al. Molecular Mechanism of Tetramethylpyrazine Ameliorating Neuroexcitotoxicity through Activating the PKA/CREB Signaling Pathway. Biomed Res Int. 2022;2022:2812839Read more>>
- Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292Read more>>
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- Shiqing Sun. et al. Pharmacodynamic structure of deer antler base protein and its mammary gland hyperplasia inhibition mechanism by mediating Raf-1/MEK/ERK signaling pathway activation. FOOD FUNCT. 2023 MaRead more>>
- Jiajun Chen. et al. Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism. J PHARM ANAL. 2023 AugRead more>>
- Yanfei Shi. et al. Naringenin promotes the expression of oxidized myofibers via the PKA signaling pathway in C57BL/6J mice and C2C12 cells. J FUNCT FOODS. 2023 Dec;111:105902Read more>>
Jurkat lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (bs-0520R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.