bs-0523R [Primary Antibody]
SOX2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SOX2

Immunogen Range: 227-317/317


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20674

Swiss Prot: P48432

Source: KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)
ICC(1:100)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse
Rat
Goat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
  • Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell?Cassociated genes in colorectal cancer."Medical Oncology 30.1 (2013): 1-11.(Read more>>
  • Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014).Read more>>
  • Wang, Li, et al. "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells."Tumor Biology (2015): 1-15.Read more>>
  • Yan, Yu-hui, et al. "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." Neurochemical Research (2016): 1-8.Read more>>
  • Liu, Yi‐Zhen, et al. "A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens." Cancer cytopathology 122.11 (2014): 833-841.Read more>>
  • Ma, Fanglin, et al. "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." Scientific Reports 6 (2016): 38805.Read more>>
  • Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106Read more>>
  • Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/3 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.Read more>>
  • Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20.Read more>>
  • Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
  • Wang L et al. Lung CSC\derived exosomal miR\210\3p contributes to a pro\metastatic phenotype in lung cancer by targeting FGFRL1. J Cell Mol Med. 2020 Jun;24(11):6324-6339.Read more>>
  • Yun Gou. et al. Homocysteine restrains hippocampal neurogenesis in focal ischemic rat brain by inhibiting DNA methylation. Neurochem Int. 2021 Jul;147:105065Read more>>
  • Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
VALIDATION IMAGES

NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody (bs-0523R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.


Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 32kD. Observed band size: 32kD.


Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:400 overnight at 4°C, DAB staining.


U251 cells (black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX2 Antibody(bs-0523R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).