DATASHEET
Host:
Rabbit
Target Protein:
SOX2
Immunogen Range:
227-317/317
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
20674
Swiss Prot:
P48432
Source:
KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Conjugation:
ALEXA FLUOR® 647
Excitation/ Emission:
650nm/665nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
34
Human
Mouse
Rat
Goat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
- Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell?Cassociated genes in colorectal cancer."Medical Oncology 30.1 (2013): 1-11.(Read more>>
- Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014).Read more>>
- Wang, Li, et al. "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells."Tumor Biology (2015): 1-15.Read more>>
- Yan, Yu-hui, et al. "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." Neurochemical Research (2016): 1-8.Read more>>
- Liu, Yi‐Zhen, et al. "A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens." Cancer cytopathology 122.11 (2014): 833-841.Read more>>
- Ma, Fanglin, et al. "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." Scientific Reports 6 (2016): 38805.Read more>>
- Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106Read more>>
- Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/3 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.Read more>>
- Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20.Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Wang L et al. Lung CSC\derived exosomal miR\210\3p contributes to a pro\metastatic phenotype in lung cancer by targeting FGFRL1. J Cell Mol Med. 2020 Jun;24(11):6324-6339.Read more>>
- Yun Gou. et al. Homocysteine restrains hippocampal neurogenesis in focal ischemic rat brain by inhibiting DNA methylation. Neurochem Int. 2021 Jul;147:105065Read more>>
- Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
- Penggang Liu. et al. Expression of tumour transcription factor GLI1 in canine mammary tumours tissue. VET MED SCI. 2022 Jun 06Read more>>
VALIDATION IMAGES
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,pemeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX2 Polyclonal Antibody(bs-0523R-AF647) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).