bs-0525R [Primary Antibody]
Thrombomodulin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Thrombomodulin

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21824

Source: KLH conjugated synthetic peptide derived from mouse Thrombomodulin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Thrombomodulin, TM is cell surface glycoprotein; plays an important role in the protein C anticoagulant pathway. It located in a vein, artery and capillary endothelial cells on the surface of plasma membrane protein. It is generally believed: TM vascular endothelial injury is an important parameter is the thrombin receptor, known in a variety of normal human tissues, can also be expressed in many tumors, TM may be similar to the E-cadherin,and is a lectin Like activity of a new class of members of the cell adhesion molecules.CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high-affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ikezoe et al. The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective function and prevents SOS in a murine model. (2017) Bone.Marrow.Transplant. 52:73-79Read more>>
  • Weifeng Hu. et al. Bone marrow-derived mesenchymal stem cells transplantation attenuates renal fibrosis following acute kidney injury by repairing the peritubular capillaries. Exp Cell Res. 2022 Feb;411:112983Read more>>
  • Juin-Hong Cherng. et al. Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds. J PERS MED. 2022 Jul;12(7):1089Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat kidney labeled with Anti-Thrombomodulin/CD141 Polyclonal Antibody, Unconjugated (bs-0525R) at 1:300 followed by conjugation to the secondary antibody and DAB staining


L1 rat heart, L2 rat brain lysates probed (bs-0525R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 61kDa.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.