bs-0529R [Primary Antibody]
IL-18 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-18

Immunogen Range: 51-120/192


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29197

Source: KLH conjugated synthetic peptide derived from rat IL-18

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells.This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.Read more>>
  • Duan S et al. NLRP3 inflammasome activation is associated with PM2. 5‐induced cardiac functional and pathological injury in mice. Environ Toxicol. 2019 Jul 16. Read more>>
  • Wu Z et al. Pyroptosis engagement and bladder urothelial cells derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after UPEC infection. Am J Physiol Cell Physiol. 2019 Jun 26.Read more>>
  • Gao M et al. Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo (a) pyrene and lipopolysaccharide? Ecotoxicol Environ Saf. 2019 Sep 24;185:109687. Read more>>
  • Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.Read more>>
  • Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. Read more>>
  • Kong D et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Research Square. 17 February 2020.Read more>>
  • Shao H et al. Glyburide attenuates ozone‐induced pulmonary inflammation and injury by blocking the NLRP3 inflammasome. Environ Toxicol. 2020 Mar 13.Read more>>
  • Mengli Cui et al. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol. 2020 Jul;84:106492.Read more>>
  • Yuan Changet al. NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury. J Neuroinflammation. 2020 Jul 23;17(1):219.Read more>>
  • Xiao-ling Zhang. et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology. 2021 May;84:1Read more>>
  • Kong Dejun. et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Stem Cells Int. 2020;2020:8883636Read more>>
  • Lei Xianghong. et al. Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-B/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediat Inflamm. 2020;2020:3934769Read more>>
  • Wei Yu. et al. Curcumin suppresses doxorubicin-induced cardiomyocyte pyroptosis via a PI3K/Akt/mTOR-dependent manner. Cardiovasc Diagn The. 2020 Aug; 10(4): 752C769Read more>>
  • Xiaoli Liu. et al. Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol. 2021 Mar;:1137Read more>>
  • Nicholas Andrikopoulos. et al. Inhibition of Amyloid Aggregation and Toxicity with Janus Iron Oxide Nanoparticles. Chem Mater. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
  • Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
  • Wangyu Wu. et al. Cholecalciferol pretreatment ameliorates ischemia/reperfusion-induced acute kidney injury through inhibiting ROS production, NF-_B pathway and pyroptosis. Acta Histochem. 2022 May;124:151875Read more>>
  • Weijian Fan. et al. RGD1564534 represses NLRP3 inflammasome activity in cerebral injury following ischemia-reperfusion by impairing miR-101a-3p-mediated Dusp1 inhibition. EXP NEUROL. 2022 Nov;:114266Read more>>
  • Jiayu Yu. et al. Disruption of the Intestinal Mucosal Barrier Induced by High Fructose and Restraint Stress Is Regulated by the Intestinal Microbiota and Microbiota Metabolites. MICROBIOL SPECTR. 2023 Jan 31Read more>>
  • Ruxue Jia. et al. PM2.5 induce myocardial injury in hyperlipidemic mice through ROS-pyroptosis signaling pathway. ECOTOX ENVIRON SAFE. 2023 Apr;254:114699Read more>>
  • Lihui Ni. et al. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. INT J BIOL MACROMOL. 2023 Jul;242:12475Read more>>
  • Yang S. Y.. et al. Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway. B EXP BIOL MED+. 2023 May;:1-6Read more>>
  • Tian Jihua. et al. SIRT1 slows the progression of lupus nephritis by regulating the?NLRP3 inflammasome through ROS/TRPM2/Ca2+ channel. CLIN EXP MED. 2023 Jun;:1-14Read more>>
  • Jihua Tian. et al. S1P/S1PR1 axis promotes macrophage M1 polarization through NLRP3 inflammasome activation in Lupus nephritis. MOL IMMUNOL. 2023 Aug;160:55Read more>>
  • Chenchen Yang. et al. Study on the mechanism of NLRP3 effect on the skeleton of de-ovalized mice. Biochemistry and Biophysics Reports. 2023 Sep;35:101496Read more>>
  • Yi Jing. et al. Polypeptide from Moschus Suppresses Lipopolysaccharide-Induced Inflammation by Inhibiting NF- B-ROS/NLRP3 Pathway. CHIN J INTEGR MED. 2023 Aug;:1-1Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-IL-18 Polyclonal Antibody (bs-0529R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining