bs-0545R-Biotin [Conjugated Primary Antibody]
SCF Polyclonal Antibody, Biotin Conjugated
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Host: Rabbit

Target Protein: SCF

Immunogen Range: 155-209/273


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4254

Swiss Prot: P21583

Source: KLH conjugated synthetic peptide derived from human SCF

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Goat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Goldstein, Bradley J., et al. "Adult c‐Kit (+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons." Journal of Comparative Neurology (2014).Read more>>
  • Wang, Li, et al. "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells."Tumor Biology (2015): 1-15.Read more>>
  • Lin, Xue-Lian, et al. "NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF." PloS one 11.2 (2016): e0149031.Read more>>
  • Yin et al. Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. (2018) Int.J.Mol.Med. 41:649-658Read more>>
  • Choo E et al. The c-kit Receptor Tyrosine Kinase Marks Sweet or Umami Sensing T1R3 Positive Adult Taste Cells in Mice. Chemosensory Perception. 2020.Read more>>
  • Xi Huaming. et al. Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. Biol Reprod. 2021 AugRead more>>
  • Huaming Xi. et al. FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. Mol Cell Endocrinol. 2021 Nov;:111505Read more>>
  • Hongjun Kuang. et al. Electroacupuncture Improves Intestinal Motility through Exosomal miR-34c-5p Targeting SCF/c-Kit Signaling Pathway in Slow Transit Constipation Model Rats.. EVID-BASED COMPL ALT. 2022 Sep;2022:8043841-8043841Read more>>
  • Huaming Xi. et al. FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway. THERIOGENOLOGY. 2023 Jan;195:103Read more>>
  • Ozge Goktepe. et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats. FOOD CHEM TOXICOL. 2023 Jul;177:113816Read more>>
  • Di Zhang. et al. Effects of Banxia Xiexin Decoction on apoptosis of interstitial cells of Cajal via regulation of MiR-451-5p: An in vivo and in vitro study. J ETHNOPHARMACOL. 2023 Oct;314:116606Read more>>
  • Karaman Enes. et al. Protective Effects of Boric Acid Taken in Different Ways on Experimental Ovarian ?schemia and Reperfusion. BIOL TRACE ELEM RES. 2023 Sep;:1-14Read more>>
  • Erol Suleyman. et al. In vitro evaluation of exocytosis-associated SNARE molecules in human granulosa cells in polycystic ovary syndrome. J ASSIST REPROD GEN. 2023 Nov;:1-13Read more>>
  • Lei Wu. et al. Naringenin Promotes Gastrointestinal Motility in Mice by Impacting the SCF/c-Kit Pathway and Gut Microbiota. FOODS. 2024 Jan;13(16):252Read more>>
VALIDATION IMAGES