bs-0547R [Primary Antibody]
Endostatin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: COL18A1

Immunogen Range: 1581-1680/1754


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 80781

Swiss Prot: P39060

Source: KLH conjugated synthetic peptide derived from human COL18A1/Endostatin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 20/190


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • GAO, DAN, et al. "Effect of peritoneal dialysis on expression of vascular endothelial growth factor, basic fibroblast growth factor and endostatin of the peritoneum in peritoneal dialysis patients." Nephrology 16.8 (2011): 736-742.Read more>>
  • Hu, Ming‑Ming, et al. "Clinical significance of serum and tumor tissue endostatin evaluation in operable non‑small cell lung cancer." Biomedical Reports.Read more>>
  • Imoto, Keisuke, et al. "Endostatin is protective against monocrotaline-induced right heart disease through the inhibition of T-type Ca2+ channel."Pflügers Archiv-European Journal of Physiology (2016): 1-12.Read more>>
  • Duan CY et al. Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells. Cell Mol Biol Lett. 2019 Apr 4;24:3. Read more>>
  • Heng Jiang. et al. miR-9 promotes canine endothelial-like cell migration by targeting COL15A1. VET MED SCI. 2023 Dec;10(1):e1339Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Endostatin Polyclonal Antibody (bs-0547R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Liver; Probed with Endostatin Polyclonal Antibody, Unconjugated (bs-0547R) at 1:500, overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.