bs-0549R [Primary Antibody]
Collagen III Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: Collagen III

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1281

Swiss Prot: P02461

Source: KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Predicted Molecular Weight: 117


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445.Read more>>
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  • Velázquez, Kandy T., et al. "miR155 deficiency aggravates high‐fat diet‐induced adipose tissue fibrosis in male mice." Physiological Reports 5.18 (2017): e13412.Read more>>
  • Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
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  • Shang L et al. Elevated β1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis. Frontiers in Physiology,(2020) 11.Read more>>
  • Xiaoxiao Zhang. et al. Effect of Guanxin V in animal model of acute myocardial infarction. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
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  • Wei P. et al. Nintedanib ameliorates tracheal stenosis by activating HDAC2 and suppressing IL-8 and VEGF in rabbit.. Am J Transl Res. 2020 Aug;12(8):4739-4748Read more>>
  • Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609Read more>>
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VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates probed with Collagen 3 Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Abdominal Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Collagen III polyclonal antibody, unconjugated (bs-0549R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.