bs-0563R
[Primary Antibody]
MDR1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MDR1
Immunogen Range: 21-100/1272
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 18671
Swiss Prot: P21447
Source: KLH conjugated synthetic peptide derived from mouse MDR1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 141
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wen-Qing, Hu, Peng Chun-Wei, and Li Yan. "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." Journal of Experimental & Clinical Cancer Research 28 (2009).Read more>>
- Peng, Lei, et al. "Development of a novel orthotopic non-small cell lung cancer model and therapeutic benefit of 2’-(2-bromohexadecanoyl)-docetaxel conjugate nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine (2014).Read more>>
- Wei et al. The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28. (2015) J.Exp.Clin.Cancer.Re. 34:64Read more>>
- Sun et al. MicroRNA-186 induces sensitivity of ovarian cancer cells to paclitaxel and cisplatin by targeting ABCB1. (2015) J.Ovarian.Re. 8:80Read more>>
- Liu et al. Chronic inflammation up-regulates P-gp in peripheral mononuclear blood cells via the STAT3/Nf-κb pathway in 2,4,6-trinitrobenzene sulfonic acid-induced colitis mice. (2015) Sci.Re. 5:13558Read more>>
- Chen et al. Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (2016) Int.J.Oncol. 48:2063-70Read more>>
- Liu QH et al. Reduced expression of annexin A1 promotes gemcitabine and 5-fluorouracil drug resistance of human pancreatic cancer. Invest New Drugs. 2019 May 23.Read more>>
- Yuan P et al. WS2 Nanosheets at Noncytotoxic Concentrations Enhance the Cytotoxicity of Organic Pollutants by Disturbing the Plasma Membrane and Efflux Pumps. Environ Sci Technol. 2020 Feb 4;54(3):1698-1709.Read more>>
- Hiroshi Kikuchiet al. Chemotherapy-Induced IL8 Upregulates MDR1/ABCB1 in Tumor Blood Vessels and Results in Unfavorable Outcome. Cancer Res. 2020 Jul 15;80(14):2996-3008.Read more>>
- Mengyao Liuet al. Paclitaxel and quercetin co-loaded functional mesoporous silica nanoparticles overcoming multidrug resistance in breast cancer. Colloids Surf B Biointerfaces. 2020 Dec;196:111284.Read more>>
- Kai-Fu Chang. et al. Cedrol, a Sesquiterpene Alcohol, Enhances the Anticancer Efficacy of Temozolomide in Attenuating Drug Resistance via Regulation of the DNA Damage Response and MGMT Expression. J Nat Prod. 2020;83(10):3021C3029Read more>>
- Xue Zhou. et al. Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells. Leukemia Lymphoma. 2020;61(14):3451-3459Read more>>
- Haruna Makita. et al. Xenografts derived from patients with head and neck cancer recapitulate patient tumour properties. Oncol Lett. 2021 May;21(5):1-1Read more>>
- Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586Read more>>
- Chi-Yen Liang. et al. Patchouli alcohol induces G0/G1 cell cycle arrest and apoptosis in vincristine-resistant non-small cell lung cancer through ROS-mediated DNA damage. THORAC CANCER. 2023 MayRead more>>
- Jinhua Gu. et al. Deciphering the mechanism of Peptostreptococcus anaerobius-induced chemoresistance in colorectal cancer: the important roles of MDSC recruitment and EMT activation. FRONT IMMUNOL. 2023; 14: 1230681Read more>>
- Jialing Sun. et al. Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology. J ETHNOPHARMACOL. 2024 Feb;:117985Read more>>
VALIDATION IMAGES
Hela cells probed with MDR1 Polyclonal Antibody, Unconjugated (bs-0563R) for 30 minutes followed by incubation with a conjugated secondary (FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Rat liver lysates probed with MDR1 Polyclonal Antibody, Unconjugated (bs-0563R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human 293T cell lysates probed with MDR1 Polyclonal Antibody, Unconjugated (bs-0563R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (bs-0563R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.