bs-0578R [Primary Antibody]
Pro-Collagen 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Collagen 1

Specificity: This antibody is produced using a peptide that is found in the C-terminal propeptide region.

Immunogen Range: 1321-1400/1464


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1277

Source: KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Type I collagen levels have been linked to bone growth and formation. Levels of the C-terminal propeptide are indicative of collagen production in vivo. Elevated levels of C-terminal propeptide have been shown in diseases associated with high levels of bone turnover, including Pagets disease, hyperthyroidism, primary hyperparathyroidism and renal osteodystrophy. Slightly elevated levels of C-terminal propeptide have also been documented in some women in early menopause.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 130


Cross Reactive Species: Human
Mouse
Rat
Dog
Rabbit
Fish

Predicted Cross Reactive Species: Cow
Sheep
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Fan, et al. "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." Stem Cells 29.6 (2011): 981-991.Read more>>
  • Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013).Read more>>
  • Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722.Read more>>
  • Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).Read more>>
  • Zhang, Qiang-Ye, et al. "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." BMC surgery 11.1 (2011): 5.Read more>>
  • Zhou, Nan, et al. "Involvement of Imperatorin Derivative‐OW1 in the antihypertensive effect and vascular remodeling of renovascular hypertension rats." Clinical and Experimental Pharmacology and Physiology (2014).Read more>>
  • Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016).Read more>>
  • Gao, Yuanyuan, et al. "TGF‐β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway." Cell biology international (2016).Read more>>
  • Chen, Cheng, et al. "Effects of Cytoskeleton Modulation on the Phenotype of Articular Chondrocytes in Monolayer and Pellet Culture." Journal of Biomaterials and Tissue Engineering 5.12 (2015): 942-950.Read more>>
  • Çakir-Özkan, Nilüfer, et al. "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." Journal of Craniofacial Surgery 27.3 (2016): 615-620.Read more>>
  • Cecen, Berivan, et al. "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." Materials Science and Engineering: C(2016).Read more>>
  • Ren, Xiang, et al. "Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient."BMC Musculoskeletal Disorders 17.1 (2016): 1.Read more>>
  • Tian, Fa‑Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine.Read more>>
  • Yang, Ning, et al. "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." World J Gastroenterol 23.7 (2017): 1203-1214.Read more>>
  • Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
  • Wang et al. The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring. (2014) Biomed.Res.In. 2014:419805Read more>>
  • Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56Read more>>
  • Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313Read more>>
  • Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344Read more>>
  • Wang Y et al. ASIC1a promotes high glucose and PDGF-induced hepatic stellate cell activation by inducing autophagy through CaMKKβ/ERK signaling pathway. (2018) Toxicol Lett. (18)31963-5Read more>>
  • Kara A et al. Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019 Feb 20;130:266-279. Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
  • Li Y et al. Klotho recovery by genistein via promoter histone acetylation and DNA demethylation mitigates renal fibrosis in mice.J Mol Med (Berl). 2019 Apr;97(4):541-552.Read more>>
  • Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. Read more>>
  • Jiang B et al. Expression of specificity protein 1 and collagen I in primary pterygial tissues. Arq Bras Oftalmol. 2019 Jun 3. Read more>>
  • Hendrijantini N et al. Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone. Eur J Dent. 2019 Feb;13(1):58-63. Read more>>
  • Chen Y et al. An improved cellular enucleation method with extracellular matrix and colchicine facilitates the study of nucleocytoplasmic interaction. Eur J Cell Biol. 2019 Sep 17:151045. Read more>>
  • Hou LS et al. Rutin mitigates hepatic fibrogenesis and inflammation through targeting TLR4 and P2X7 receptor signaling pathway in vitro and in vivo. Journal of Functional Foods,(2019),103700.Read more>>
  • Wu W et al. Yu Jin Pulvis inhibits carbon tetrachloride-induced liver fibrosis by blocking the MAPK and PI3K/Akt signaling pathways. Am J Transl Res. 2019 Sep 15;11(9):5998-6006. eCollection 2019.Read more>>
  • Luo P et al. IL‐37b alleviates inflammation in the temporomandibular joint cartilage via IL‐1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. Read more>>
  • Wang T et al. The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Mol Med Rep. 2020 Mar;21(3):1097-1106. Read more>>
  • Zhang X et al. Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling.Biochem Cell Biol. 2018 Dec;96(6):742-751. Read more>>
  • Motoji H et al. Vitamin C and eggshell membrane facilitate orthodontic tooth movement and induce histological changes in the periodontal tissue. J Oral Biosci. 2020 Feb 4. pii: S1349-0079(20)30006-2.Read more>>
  • Yang KC et al. Effects of scaffold geometry on chondrogenic differentiation of adipose-derived stem cells. Materials Science and Engineering: C, 110733.Read more>>
  • Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachlorideCinduced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204Read more>>
  • Wang Y et al. Metformin attenuates TGF-1-induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF- pathways. 3 Biotech. 2020 Jun;10(6):287.Read more>>
  • Yihang Yuet al. Human umbilical cord mesenchymal stem cell attenuates renal fibrosis via TGF-/Smad signaling pathways in vivo and in vitro. Eur J Pharmacol. 2020 Sep 15;883:173343.Read more>>
  • Xiajie Linet al. Leucine-activated nanohybrid biofilm for skin regeneration via improving cell affinity and neovascularization capacity. J Mater Chem B. 2020 Sep 21;8(35):7966-7976.Read more>>
  • Jian Songet al. 20S-Protopanaxatriol Ameliorates Hepatic Fibrosis, Potentially Involving FXR-Mediated Inflammatory Signaling Cascades. J Agric Food Chem. 2020 Aug 5;68(31):8195-8204.Read more>>
  • Min Jianget al. P2X7R orchestrates the progression of murine hepatic fibrosis by making a feedback loop from macrophage to hepatic stellate cells. Toxicol Lett. 2020 Oct 15;333:22-32.Read more>>
  • Deng Z et al. Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes\associated protein. J Cell Mol Med. 2020 Jul;24(14):8179-8193.Read more>>
  • Tongtong Song. et al. Dynamic modulation of gut microbiota improves post\myocardial infarct tissue repair in rats via butyric acid\mediated histone deacetylase inhibition. Faseb J. 2021 Mar;35(3):e21385Read more>>
  • Xiaoxiao Zhang. et al. Effect of Guanxin V in animal model of acute myocardial infarction. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
  • Chun-Sheng Shi. et al. Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues. Int J Ophthalmol-Chi. 2021; 14(2): 223C227Read more>>
  • Hao H. et al. Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.. Aging-Us. 2021 Jan;13(2):2982-3009Read more>>
  • Chen-Chie Wang. et al. Infrapatellar Fat PadsCDerived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold:. Cartilage. 0;()Read more>>
  • Jiahui Fu. et al. Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration. Front Bioeng Biotech. 2020; 8: 595096Read more>>
  • Maolin Zhang. et al. Recapitulation of cartilage/bone formation using iPSCs via biomimetic 3D rotary culture approach for developmental engineering. Biomaterials. 2020 Nov;260:120334Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:300 followed by conjugation to the secondary PE antibody and DAB staining


Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Pro-Collagen 1 Polyclonal Antibody, Unconjugated (bs-0578R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.