bs-0611R [Primary Antibody]
Adiponectin receptor 2 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Adiponectin receptor 2

Specificity: This antibody may cross react with Adiponectin receptor 1 due to sequence similarity.

Immunogen Range: 315-340/386


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79602

Swiss Prot: Q86V24

Source: KLH conjugated synthetic peptide derived from human Adiponectin receptor 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Su, et al. "The ameliorating effect of rosiglitazone on experimental nonalcoholic steatohepatitis is associated with regulating adiponectin receptor expression in rats." European journal of pharmacology 650.1 (2011): 384.Read more>>
  • Ji, Hongfei, et al. "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." Molecular Biology Reports: 1-9.Read more>>
  • Morad, Vivian, Annelie Abrahamsson, and Charlotta Dabrosin. "Estradiol affects extracellular leptin: adiponectin ratio in human breast tissue in vivo." The Journal of Clinical Endocrinology & Metabolism (2014).Read more>>
  • Hsu, Ying-Jung, et al. "Effects of Fenofibrate on Adiponectin Expression in Retinas of Streptozotocin-Induced Diabetic Rats." Journal of diabetes research 2014 (2014).Read more>>
  • Zhou et al. Associations of adiponectin receptor 2 (AdipoR2) gene polymorphisms and AdipoR2 protein expression levels with the risk of colorectal cancer: A case-control study. (2017) Mol.Med.Rep. 16:3983-3993Read more>>
  • Guo et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. (2012) Cardiovasc.Diabetol. 11:94Read more>>
  • Annamria Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465Read more>>
  • Guoying Zhu. et al. Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats. HELIYON. 2022 Jul;8:e09872Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:300, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Skeletal muscle lysates; Lane 5: Human 293T cell lysates probed with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.