DATASHEET
Host:
Rabbit
Target Protein:
ZNF300
Immunogen Range:
165-250/604
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
91975
Swiss Prot:
Q96RE9
Source:
KLH conjugated synthetic peptide derived from human ZNF300
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms.
PRODUCT SPECIFIC PUBLICATIONS
- Xue et al. ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site. (2010) Cell.Mol.Biol.Lett. 15:530-40Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ZNF300 Polyclonal Antibody, Unconjugated (bs-0628R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 mouse intestine lysates probed with Anti ZNF300 Polyclonal Antibody, Unconjugated (bs-0628R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size:66kD.\n