bs-0630R [Primary Antibody]
Angiotensin II Type 1 Receptor Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Angiotensin II Type 1 Receptor

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 185

Swiss Prot: P30556

Source: KLH conjugated synthetic peptide derived from human AT1R

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kusumawati et al. Inhibitory Effect of the Punica granatum Fruit Extract on Angiotensin-II Type I Receptor and Thromboxane B2 in Endothelial Cells Induced by Plasma from Preeclamptic Patients. (2016) Adv.Prev.Me. 2016:6028989Read more>>
  • Chen Q et al. Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. Biomed Pharmacother. 2019 Jul;115:108971. Read more>>
  • Liu J et al. Sini decoction alleviates E. coli induced acute lung injury in mice via equilibrating ACE-AngII-AT1R and ACE2-Ang-(1-7)-Mas axis.Life Sci. 2018 Sep 1;208:139-148.Read more>>
  • Hongxu Bao. et al. Exposure to Real-Ambient Particulate Matter Induced Vascular Hypertrophy by Activation of PDGFR. J HAZARD MATER. 2023 Feb;:130985Read more>>
  • Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1C7) axis via NF-B/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated (bs-0630R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human pneumonic lung tissue labeled with Anti-EDG4/LPA2 Polyclonal Antibody, Unconjugated (bs-2881R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Mouse kidney lysates probed with Angiotensin II Type 1 Receptor Polyclonal Antibody, Unconjugated (bs-0630R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-0630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-0630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.