bs-0636R [Primary Antibody]
Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody
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Host: Rabbit

Target Protein: Phospho-P38 MAPK (Thr180 + Tyr182)

Modification Site: Thr180 + Tyr182

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1432

Source: KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Rosenzweig, Derek H., et al. "Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis." Osteoarthritis and Cartilage (2012).Read more>>
  • Kumar et al. Simultaneous targeting of 5-LOX-COX and ODC block NNK-induced lung adenoma progression to adenocarcinoma in A/J mice. (2016) Am.J.Cancer.Re. 6:894-909Read more>>
  • Xing et al. Increased expression of receptor for advanced glycation end-products worsens focal brain ischemia in diabetic rats. (2012) Neural.Regen.Res. 7:1000-5Read more>>
  • Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257Read more>>
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • Song G et al. Chrysophanol attenuates airway inflammation and remodeling through nuclear factor‐kappa B signaling pathway in asthma. Phytother Res. 2019 Jul 17. Read more>>
  • Deng Z et al. M1 macrophage mediated increased reactive oxygen species (ROS) influence wound healing via the MAPK signaling in vitro and in vivo. Toxicol Appl Pharmacol. 2019 Mar 1;366:83-95.Read more>>
  • Shi X et al. Botulinum toxin type A ameliorates adjuvant-arthritis pain by inhibiting microglial activation-mediated neuroinflammation and intracellular molecular signaling. Toxicon. 2019.Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.Read more>>
  • Zhao Z et al. Lactobacillus plantarum NA136 ameliorates nonalcoholic fatty liver disease by modulating gut microbiota, improving intestinal barrier integrity, and attenuating inflammation. Appl Microbiol Biotechnol. 2020 Apr 26. Read more>>
  • Liu A et al. Cimifugin ameliorates imiquimod-induced psoriasis by inhibiting oxidative stress and inflammation via NF-B/MAPK pathway. Biosci Rep. 2020 Jun 26;40(6):BSR20200471.Read more>>
  • Qiumei Shi. et al. Echinacea polysaccharide attenuates lipopolysaccharide?induced acute kidney injury via inhibiting inflammation, oxidative stress and the MAPK signaling pathway. Int J Mol Med. 2021 Jan;47(1):243-255Read more>>
  • Jingwen Zhou. et al. Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-B) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways. Med Sci Monitor. 2021; 27: e929438-1Ce929438-12Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Xiuhong Wang. et al. Protective effect of combination of anakinra and MCC950 against acute lung injury is achieved through suppression of the NF-B-mediated-MAPK and NLRP3-caspase pathways. Int Immunopharmacol. 2021 Aug;97:107506Read more>>
  • Lili Liu. et al. Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-B Pathway. 2021 Jun 06Read more>>
  • Yaping Wang. et al. Blockade of GITRL/GITR signaling pathway attenuates house dust mite-induced allergic asthma in mice through inhibition of MAPKs and NF-B signaling. Mol Immunol. 2021 Sep;137:238Read more>>
  • Li, Muzhe. et al. Effects of adenovirus-mediated knockdown of IRAK4 on synovitis in the osteoarthritis rabbit model. Arthritis Res Ther. 2021 Dec;23(1):1-12Read more>>
  • Junfeng Ke. et al. CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway. Int J Mol Sci. 2021 Jan;22(22):12229Read more>>
  • Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
  • Zhang, Rongrong. et al. Compound traditional Chinese medicine dermatitis ointment ameliorates inflammatory responses and dysregulation of itch-related molecules in atopic dermatitis. Chin Med-Uk. 2022 Dec;17(1):1-19Read more>>
  • Linyan Cheng. et al. Icariin attenuates thioacetamide_induced bone loss via the RANKL_p38/ERK_NFAT signaling pathway. Mol Med Rep. 2022 Apr;25(4):1-11Read more>>
  • Yudan Zhao. et al. COX-2 is required to mediate crosstalk of ROS-dependent activation of MAPK/NF-_B signaling with pro-inflammatory response and defense-related NO enhancement during challenge of macrophage-like cell line with Giardia duodenalis. PLOS NEGLECT TROP D. 2022 Apr;16(4):e0010402Read more>>
  • Yanjie Wang. et al. Bisdemethoxycurcumin attenuates OVA_induced food allergy by inhibiting the MAPK and NF__B signaling pathways. EXP THER MED. 2022 Jun;23(6):1-8Read more>>
  • Chao Li. et al. Research on the effect and underlying molecular mechanism of Cangzhu in the treatment of gouty arthritis. EUR J PHARMACOL. 2022 Jul;927:175044Read more>>
  • Jian Kang. et al. Ginsenoside Rg1 Mitigates Porcine Intestinal Tight Junction Disruptions Induced by LPS through the p38 MAPK/NLRP3 Inflammasome Pathway. TOXICS. 2022 Jun;10(6):285Read more>>
  • Zhang, Di. et al. Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-_B and MAPK signaling pathways. BMC VET RES. 2022 Dec;18(1):1-11Read more>>
  • Xiaobao Gong. et al. Synthesis and anti-inflammatory activity of paeonol derivatives with etherized aryl urea by regulating TLR4/MyD88 signaling pathway in RAW264.7 cell. BIOORG CHEM. 2022 Oct;127:105939Read more>>
  • Myung-Ju Lee. et al. CXCL1 confers a survival advantage in Kaposi's sarcoma-associated herpesvirus-infected human endothelial cells through STAT3 phosphorylation. J MED VIROL. 2022 JulRead more>>
  • Li Xu. et al. Fenpropathrin increases gliquidone absorption via causing damage to the integrity of intestinal barrier. ECOTOX ENVIRON SAFE. 2022 Sep;242:113882Read more>>
  • Torigoe, Miki. et al. Hydroxychloroquine suppresses anti-GBM nephritis via inhibition of JNK/p38 MAPK signaling. CLIN EXP NEPHROL. 2022 Oct;:1-12Read more>>
  • Honghong Zhan. et al. Oxybaphus himalaicus Mitigates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/MD2 Complex Formation. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2307Read more>>
  • Yinxi Yang. et al. Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. ONCOL LETT. 2022 Dec;24(6):1-11Read more>>
  • Jin Chen. et al. Surface functionalization-dependent inflammatory potential of polystyrene nanoplastics through the activation of MAPK/ NF-B signaling pathways in macrophage Raw 264.7. ECOTOX ENVIRON SAFE. 2023 Feb;251:11452Read more>>
  • Ma, Dufang. et al. Astragalus polysaccharide prevents heart failure-induced cachexia by alleviating excessive adipose expenditure in white and brown adipose tissue. LIPIDS HEALTH DIS. 2023 Dec;22(1):1-16Read more>>
  • Yi Yan. et al. Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis. Advanced Science. 2023 Feb;:220749Read more>>
  • Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1C7) axis via NF-B/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11Read more>>
  • Hao Huang. et al. MAP4K4 is involved in the neuronal development of retinal photoreceptors. EXP EYE RES. 2023 Aug;233:109524Read more>>
  • Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
  • Qi Han. et al. Co-exposure to polystyrene microplastics and di-(2-ethylhexyl) phthalate aggravates allergic asthma through the TRPA1-p38 MAPK pathway. TOXICOL LETT. 2023 Aug;384:73Read more>>
  • Huan Hou. et al. Osthole protects H9c2 cardiomyocytes against trastuzumab-induced damage by enhancing autophagy through the p38MAPK/mTOR signaling pathway. TOXICOL IN VITRO. 2023 Dec;93:105704Read more>>
  • Keyi Nong. et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. INT IMMUNOPHARMACOL. 2024 Mar;130:111757Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-P38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated(bs-0636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.