bs-0637R [Primary Antibody]
P38 MAPK Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P38 MAPK

Immunogen Range: 141-240/360

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1432

Swiss Prot: Q16539

Source: KLH conjugated synthetic peptide derived from human P38MAPK

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Human
Mouse
Rat
Rabbit
Others

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Lingrui, et al. "Nrf2/ARE pathway activation, HO-1 and NQO1 induction by polychlorinated biphenyl quinone is associated with reactive oxygen species and PI3K/AKT signaling." Chemico-Biological Interactions (2013).Read more>>
  • Wang, B., et al. "Ginsenoside Rd inhibits apoptosis following spinal cord ischemia/reperfusion injury." Neural Regeneration Research 9.18 (2014): 1678.Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates lung and liver injuries in sepsis."International Immunopharmacology 30 (2016): 50-56.Read more>>
  • Cong, Lin, and Wenting Chen. "Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats." Basic & Clinical Pharmacology & Toxicology(2016).Read more>>
  • Shi, Zeyu, et al. "Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice." Biological Trace Element Research (2016): 1-6.Read more>>
  • Zhang, Nan, et al. "Effect of vasopressin on hippocampal injury in a rodent model of asphyxial cardiopulmonary arrest." Experimental and therapeutic medicine 11.4 (2016): 1385-1392.Read more>>
  • Zhang, Jianfei, et al. "Peroxiredoxin 1 has an anti-apoptotic role via apoptosis signal-regulating kinase 1 and p38 activation in mouse models with oral precancerous lesions." Oncology Letters.Read more>>
  • Zhao, Haiyan, et al. "Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension." International Immunopharmacology 42 (2017): 115-121.Read more>>
  • Ning, Chong, et al. "Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro." Molecular Nutrition & Food Research (2017).Read more>>
  • Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).Read more>>
  • Yu, Wu, et al. "BEX4 upregulation alters Sertoli cell growth properties and protein expression profiles: An explanation for cadmium‐induced testicular Sertoli cell injury." Journal of Biochemical and Molecular Toxicology (2017).Read more>>
  • Li, Jing, et al. "Downregulation of BRAF‑activated non‑coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells." Oncology Letters 14.4 (2017): 4751-4757.Read more>>
  • Liu, Xinwei, et al. "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." Molecular Medicine Reports 16.1 (2017): 192-200.Read more>>
  • Zhao, Dongmei, et al. "Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro." Experimental and Therapeutic Medicine.Read more>>
  • Li, Mengdi, et al. "Selenoprotein K Mediates the Proliferation, Migration, and Invasion of Human Choriocarcinoma Cells by Negatively Regulating Human Chorionic Gonadotropin Expression via ERK, p38 MAPK, and Akt Signaling Pathway." Biological Trace Element Research (2017): 1-13.Read more>>
  • Zhang et al. Peroxiredoxin 1 has an anti-apoptotic role via apoptosis signal-regulating kinase 1 and p38 activation in mouse models with oral precancerous lesions. (2016) Oncol.Let. 12:413-420Read more>>
  • Li and Ai Hydrogen saline suppresses neuronal cell apoptosis and inhibits the p38 mitogen‑activated protein kinase‑caspase‑3 signaling pathway following cerebral ischemia‑reperfusion injury. (2017) Mol.Med.Re. 16:5321-5325Read more>>
  • Li et al. Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells. (2017) Oncol.Lett. 14:4751-4757Read more>>
  • Zhao et al. Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damagein vivoandin vitro. (2017) Exp.Ther.Med. 14:5515-5522Read more>>
  • Wang et al. Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration. (2011) FEMS.Immunol.Med.Microbiol. 63:82-92Read more>>
  • Gong et al. Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling. (2018) Med.Sci.Monit. 24:3514-3523Read more>>
  • Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257Read more>>
  • Cong and Chen Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats. (2016) Basic.Clin.Pharmacol.Toxicol. Read more>>
  • Oyagbemi AA et al. Quercetin attenuates hypertension induced by sodium fluoride via reduction in oxidative stress and modulation of HSP 70/ERK/PPARγ signaling pathways. (2018) Sep 02Read more>>
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Zhuang S et al. Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs.(2019)Life Sci. Life Sci. Jan 1;216:168-175. Read more>>
  • Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161. Read more>>
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
  • Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
  • Ola-Davies OE et al. Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: involvement of Kim-1/p38 MAPK/Nrf2 signaling. J Basic Clin Physiol Pharmacol. 2019 May 3.Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Song G et al. Chrysophanol attenuates airway inflammation and remodeling through nuclear factor‐kappa B signaling pathway in asthma. Phytother Res. 2019 Jul 17. Read more>>
  • Pan LX et al. TMEM100 mediates inflammatory cytokines secretion in hepatic stellate cells and its mechanism research. Toxicol Lett. 2019 Jan 10. pii: S0378-4274(18)31704-1. Read more>>
  • Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596. Read more>>
  • Zhou J et al. Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway. Acta Histochem. 2019 Oct 3:151455. Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Zhang J et al. Down‐regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2019 Nov 17. Read more>>
  • Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.Read more>>
  • Shen L et al. Vitamin E and Vitamin C Attenuate Di-(2-ethylhexyl) Phthalate-induced Blood-Testis Barrier Disruption By p38 MAPK In Immature SD Rats.Reprod Toxicol. 2018 Oct;81:17-27. Read more>>
  • Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.Read more>>
  • Yuan L et al. Long non-coding RNA H19 protects H9c2 cells against hypoxia‑induced injury by activating the PI3K/AKT and ERK/p38 pathways. Molecular Medicine Reports, (2020). 21, 1709-1716. Read more>>
  • Liu F et al. Bioinformatic and experimental findings to indicate anti-cancer targets and mechanisms of calycosin against nasopharyngeal carcinoma. Research Square;12 February 2020.Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Zhao Z et al. Lactobacillus plantarum NA136 ameliorates nonalcoholic fatty liver disease by modulating gut microbiota, improving intestinal barrier integrity, and attenuating inflammation. Appl Microbiol Biotechnol. 2020 Apr 26. Read more>>
  • Liu B et al. MicroRNA‐379 mediates pigmentation, migration, and proliferation of melanocytes by targeting the insulin‐like growth factor 1 receptor. Exp Dermatol. 2020 May;29(5):467-476.Read more>>
  • Ji X et al. Double-component diazeniumdiolate derivatives as anti-cancer agents. Bioorg Med Chem. 2020 Apr 15;28(8):115405.Read more>>
  • Gao HE et al. Effects of lifelong exercise on age-related body composition, oxidative stress, inflammatory cytokines, and skeletal muscle proteome in rats. Mech Ageing Dev. 2020 Jul;189:111262.Read more>>
  • Liu A et al. Cimifugin ameliorates imiquimod-induced psoriasis by inhibiting oxidative stress and inflammation via NF-B/MAPK pathway. Biosci Rep. 2020 Jun 26;40(6):BSR20200471.Read more>>
  • Liu A et al. Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-B and MAPKs signaling pathwaysBioengineered.2021 Dec;12(1):183-195.Read more>>
VALIDATION IMAGES

Independently Validated Antibody, image provided by Science Exchange, badge number 029806: L1 HeLa cell lysate, L2 C6/36 cell lysates probed with Anti-P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:100000 for 90 min at 37˚C. Predicted band 41kDa. A strong band was observed in the positive control sample at the correct molecular weight.


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti p38MAPK/MAPK14/p38Alpha Polyclonal Antibody, Unconjugated (bs-0637R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P38 MAPK Polyclonal Antibody(bs-0637R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (P38 MAPK) polyclonal Antibody, Unconjugated (bs-0637R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (P38 MAPK) polyclonal Antibody, Unconjugated (bs-0637R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:200 overnight at 4°C, DAB staining.