bs-0639R [Primary Antibody]
GFP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GFP

Immunogen Range: 151-238/238


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 107331116

Swiss Prot: P42212

Source: KLH conjugated synthetic peptide derived from belt jellyfish GFP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF()

Predicted Molecular Weight: 27


Cross Reactive Species: Tags
Others
(protein)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Z-G., et al. "Knockdown of Porcine Endogenous Retroviruses by RNA Interference in Chinese Experimental Miniature Pig Fibroblasts." Transplantation Proceedings. Vol. 45. No. 2. Elsevier, 2013.Read more>>
  • Radics, Julia, Lisa Königsmaier, and Thomas C. Marlovits. "Structure of a pathogenic type 3 secretion system in action." Nature structural & molecular biology (2014).Read more>>
  • Mou C et al. Identification of Nuclear Localization Signals in the ORF2 Protein of Porcine Circovirus Type 3. Viruses. 2019 Nov 22;11(12). pii: E1086. Read more>>
VALIDATION IMAGES

Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P); Lane 2: Control (-) E.coli lysates probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: GFP-tagged fusion protein probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:20000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


GFP-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-33009P) probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000~10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.