bs-0646R-BF488 [Conjugated Primary Antibody]
CD34 Polyclonal Antibody, AbBy Fluor-488 Conjugated
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Host: Rabbit

Target Protein: CD34

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 947

Swiss Prot: P28906

Source: KLH conjugated synthetic peptide derived from human CD34

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Conjugation: AbBy Fluor-488

Excitation/ Emission: 499nm/519nm

Size: 100uL

Concentration: 1mg/ml

Applications: WB(1:1000-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IP(1:20-100)
IF(1:100-500)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Erin, Nuray, et al. "Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma." Breast Cancer Research and Treatment (2013): 1-13.Read more>>
  • Zeng, Chao, et al. "Prostate-specific membrane antigen: a new potential prognostic marker of osteosarcoma." Medical Oncology 29.3 (2012): 2234-2239.Read more>>
  • Zhang, Weidong, et al. "Comparisons of Rabbit Bone Marrow Mesenchymal Stem Cell Isolation and Culture Methods In Vitro." PLOS ONE 9.2 (2014): e88794.Read more>>
  • Li, Guanghui, et al. "Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits." British Journal of Oral and Maxillofacial Surgery (2013).Read more>>
  • Yu, Jie, et al. "Activation of liver X receptor enhances the proliferation and migration of endothelial progenitor cells and promotes vascular repair through PI3K/Akt/eNOS signaling pathway activation." Vascular Pharmacology (2014).Read more>>
  • Bi, Lingyun, et al. "Effect of bone marrow stem cell mobilisation on the expression levels of cellular growth factors in a rat model of acute tubular necrosis." Experimental and Therapeutic Medicine.10(2):618-624Read more>>
  • Bi, Lingyun, et al. "Effect of bone marrow stem cell mobilisation on the expression levels of cellular growth factors in a rat model of acute tubular necrosis." Experimental and Therapeutic Medicine.Read more>>
  • Sun, Wei, et al. "Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-1." (2015) Stem Cells InternationalRead more>>
  • Zhang, Q. L., et al. "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of liaoning cashmere goats." Genet Mol Res 12.12 (2013): 6424-6432.Read more>>
  • Yu, Z., et al. "Sonic hedgehog and retinoic Acid induce bone marrow-derived stem cells to differentiate into glutamatergic neural cells." Journal of immunoassay & immunochemistry 36.1 (2015): 1.Read more>>
  • Huang, Sheng‑Li, Jian Jiao, and Hong‑Wei Yan. "Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model." Experimental and therapeutic medicine11.1 (2016): 177-182.Read more>>
  • Guo, Jian-wen, et al. "Combinatorial effects of Naomai Yihao Capsules () and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." Journal of Traditional Chinese Medicine 32.1 (2012): 87-92.Read more>>
  • Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
  • Zhu et al. Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells. (2017) Mol.Med.Re. 16:3201-3207Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Chen et al. Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis. (2010) Mol.Cancer.The. 9:2164-74Read more>>
  • Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313Read more>>
  • Miwa et al. Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer. (2015) Cancer.Me. 4:1091-100Read more>>
  • Rong et al. Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro. (2015) Int.J.Clin.Exp.Patho. 8:1282-91Read more>>
  • Chang et al. Telocytes in the Spleen. (2015) PLoS.On. 10:e0138851Read more>>
  • Li et al. Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway. (2016) Oncol.Rep. 36:936-44Read more>>
  • Huang et al. Hepatitis B Virus Replication in CD34+ Hematopoietic Stem Cells From Umbilical Cord Blood. (2016) Med.Sci.Monit. 22:1673-81Read more>>
  • Zhou et al. Comparison of human adipose stromal vascular fraction and adipose-derived mesenchymal stem cells for the attenuation of acute renal ischemia/reperfusion injury. (2017) Sci.Rep. 7:44058Read more>>
  • Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
  • Sha Y et al. MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia‐induced cell apoptosis and accelerating angiogenesis.(2018) J Cell Physiol. 2018 Oct 14. Read more>>
  • Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161. Read more>>
  • Li H et al. siRNA‐mediated silencing of PAI‐1 gene acts as a promoter over the recanalization of endothelial progenitor cells in rats with venous thrombosis. J Cell Physiol. 2019 Apr 14. Read more>>
  • Jin J et al. Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.Stem Cell Res Ther. 2019 Mar 15;10(1):95. Read more>>
  • Chen X et al. Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium.Stem Cell Res Ther. 2019 Mar 21;10(1):107.Read more>>
  • Zhao F et al. Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation. Tissue Eng Part A. 2019 Jun 26.Read more>>
  • Ishiguro H et al. Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model. Acta Biomater. 2019 Mar 15;87:118-129. Read more>>
  • Zhai L et al. Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes. Stem Cell Res Ther. 2020 Jan 3;11(1):10.Read more>>
  • Kong L et al. Silencing MicroRNA-137-3p, which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis. Int J Biol Sci. 2020 Jan 14;16(4):655-670. Read more>>
  • Nau C et al. Influence of the induced membrane filled with syngeneic bone and regenerative cells on bone healing in a critical size defect model of the rat's femur.Injury. 2018 Oct;49(10):1721-1731. Read more>>
  • Dworacka M et al. Simvastatin attenuates the aberrant expression of angiogenic factors induced by glucose variability.Diabetes Res Clin Pract. 2018 Sep;143:245-253.Read more>>
  • Chen L et al. Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells. Am J Physiol Cell Physiol. 2020 May 1;318(5):C981-C990.Read more>>
  • Gao Y et al. Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Exp Ther Med. 2020 Mar;19(3):2202-2210.Read more>>
  • Mehdinia Z et al. Restoration of estrous cycles by co-transplantation of Mouseovarian tissue with MSCs. Cell Tissue Res. 2020 Sep;381(3):509-525.Read more>>
  • Mei X et al. Expression of VEGF, CD73 and their relationship with clinical pathology, microvessel density, and prognosis in renal cell carcinoma. Transl Androl Urol. 2020 Jun;9(3):1366-1373.Read more>>
  • Feng-Yen Linet al. Dipeptidyl Peptidase-4 Inhibitor Decreases Allograft Vasculopathy Via Regulating the Functions of Endothelial Progenitor Cells in Normoglycemic Rats. Cardiovasc Drugs Ther. 2020 Jul 4.Read more>>
  • Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep. 2020 Jul 24;10(1):12448.Read more>>
  • Kai-Li Liu et al. Effects of seawater immersion on open traumatic brain injury in rabbit model. Brain Res. 2020 Sep 15;1743:146903.Read more>>
  • Wu J et al. Upregulation of miRNA-9-5p promotes angiogenesis after traumatic brain injury by inhibiting Ptch-1. Neuroscience. 2020 Aug 1;440:160-174.Read more>>
  • Yihua Liu. et al. Endothelial cell adhesion and blood response to hemocompatible peptide 1 (HCP-1), REDV, and RGD peptide sequences with free N-terminal amino groups immobilized on a biomedical expanded polytetrafluorethylene surface. 2020 Nov 27Read more>>
  • Xuewen Qian. et al. Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. Lab Invest. 2020 Aug;101(3):280-291Read more>>
  • Xia Dai. et al. Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes. Cell Reprogram. 2020 Aug;22(4):189-197Read more>>
  • Ziwei Luo. et al. miR\203a\3p promotes loureirin A\induced hair follicle stem cells differentiation by targeting Smad1. Anat Rec. 2021 Mar;304(3):531-54Read more>>
  • Hongxia Wei. et al. MRI tracing of ultrasmall superparamagnetic iron oxide nanoparticle?labeled endothelial progenitor cells for repairing atherosclerotic vessels in rabbits. Mol Med Rep. 2020 Oct;22(4):3327-3337Read more>>
  • Tianli Yang. et al. Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders:. J Tissue Eng. 2021;()Read more>>
  • Mahmoud E. Youssef. et al. 7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.