bs-0647R
[Primary Antibody]
CD4 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD4
Immunogen Range: 385-457/457
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 24932
Swiss Prot: P01730
Source: KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Pig
Chicken
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Smit, Francis Edwin, et al. "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?." Cell and Tissue Banking: 1-14.Read more>>
- Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.Read more>>
- Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96Read more>>
- Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
- Hu X et al. Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus. Chemosphere. 2019 Jul 22;237:124427. Read more>>
- Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508. Read more>>
- Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11Read more>>
- Maria Angelica Cortez. et al. Bone morphogenetic protein 7 promotes resistance to immunotherapy. Nat Commun. 2020 Sep;11(1):1-14Read more>>
- Rosiris Len-Rivera. et al. Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era. Mbio. 2021 Apr;12(2):e03633-2Read more>>
- Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
- Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26Read more>>
- Nie, Ding. et al. The intestinal flora of patients with GHPA affects the growth and the expression of PD-L1 of tumor. 2021 Oct 13Read more>>
- Kousuke Umeda. et al. Transcriptomic Analysis of the Effects of Chemokine Receptor CXCR3 Deficiency on Immune Responses in the Mouse Brain during Toxoplasma gondii Infection. Microorganisms. 2021 Nov;9(11):234Read more>>
- Weidan Fang. et al. microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer. Bioengineered. 2022;13(1):1025-1038Read more>>
- Long-Yi Chan. et al. Cre/LoxP Genetic Recombination Sustains Cartilage Anabolic Factor Expression in Hyaluronan Encapsulated MSCs Alleviates Intervertebral Disc Degeneration. Biomedicines. 2022 Mar;10(3):555Read more>>
- Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 47694785Read more>>
- Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178Read more>>
- Ryohei Annen. et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model. INT J MOL SCI. 2022 Jan;23(16):9445Read more>>
- Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
- Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656Read more>>
- Prema Robinson. et al. Genetic and Small-Molecule Modulation of Stat3 in a Mouse Model of Crohn’s Disease. J CLIN MED. 2022 Jan;11(23):702Read more>>
- Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
- Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14Read more>>
- Jia-qi Yuan. et al. S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. HELIYON. 2023 Feb;9:e13294Read more>>
- Qing Yao. et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. ASIAN J PHARM SCI. 2023 Feb;:100782Read more>>
- Wenkai Ren. et al. Echinacea purpurea-derived homogeneous polysaccharide exerts anti-tumor efficacy via facilitating M1 macrophage polarization. The Innovation. 2023 Mar 13; 4(2): 100391Read more>>
- Prema Robinson. et al. Therapeutic Potential of a Small-Molecule STAT3 Inhibitor in a Mouse Model of Colitis. CANCERS. 2023 Jan;15(11):2977Read more>>
- Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
- Nahyun Choi. et al. Involvement of DKK1 secreted from adipose-derived stem cells in alopecia areata. CELL PROLIFERAT. 2023 Nov;:e13562Read more>>
VALIDATION IMAGES
Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with (bs-0647R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.
Image provided by the Independent Validation Program, badge number 029634:Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Lane 1: HL60 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Spleen; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Lymph Node; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
Human HL60 Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 1 X PBS / 2% BSA / 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with CD4 Polyclonal Antibody Unconjugated (bs-0647R; green) at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-FITC (bs-0295G-FITC), was used for 40 min at room temperature. Primary antibody staining is compared to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).
Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U937 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.