bs-0647R [Primary Antibody]
CD4 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD4

Immunogen Range: 385-457/457


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24932

Swiss Prot: P01730

Source: KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Pig
Chicken

Predicted Cross Reactive Species: Dog
Cow
Sheep
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Smit, Francis Edwin, et al. "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?." Cell and Tissue Banking: 1-14.Read more>>
  • Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.Read more>>
  • Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96Read more>>
  • Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
  • Hu X et al. Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus. Chemosphere. 2019 Jul 22;237:124427. Read more>>
  • Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508. Read more>>
  • Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11Read more>>
  • Maria Angelica Cortez. et al. Bone morphogenetic protein 7 promotes resistance to immunotherapy. Nat Commun. 2020 Sep;11(1):1-14Read more>>
  • Rosiris Len-Rivera. et al. Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era. Mbio. 2021 Apr;12(2):e03633-2Read more>>
  • Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
  • Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26Read more>>
  • Nie, Ding. et al. The intestinal flora of patients with GHPA affects the growth and the expression of PD-L1 of tumor. 2021 Oct 13Read more>>
  • Kousuke Umeda. et al. Transcriptomic Analysis of the Effects of Chemokine Receptor CXCR3 Deficiency on Immune Responses in the Mouse Brain during Toxoplasma gondii Infection. Microorganisms. 2021 Nov;9(11):234Read more>>
  • Weidan Fang. et al. microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer. Bioengineered. 2022;13(1):1025-1038Read more>>
  • Long-Yi Chan. et al. Cre/LoxP Genetic Recombination Sustains Cartilage Anabolic Factor Expression in Hyaluronan Encapsulated MSCs Alleviates Intervertebral Disc Degeneration. Biomedicines. 2022 Mar;10(3):555Read more>>
  • Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 47694785Read more>>
  • Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178Read more>>
  • Ryohei Annen. et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model. INT J MOL SCI. 2022 Jan;23(16):9445Read more>>
  • Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
  • Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656Read more>>
  • Prema Robinson. et al. Genetic and Small-Molecule Modulation of Stat3 in a Mouse Model of Crohn’s Disease. J CLIN MED. 2022 Jan;11(23):702Read more>>
  • Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
  • Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14Read more>>
  • Jia-qi Yuan. et al. S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. HELIYON. 2023 Feb;9:e13294Read more>>
  • Qing Yao. et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. ASIAN J PHARM SCI. 2023 Feb;:100782Read more>>
  • Wenkai Ren. et al. Echinacea purpurea-derived homogeneous polysaccharide exerts anti-tumor efficacy via facilitating M1 macrophage polarization. The Innovation. 2023 Mar 13; 4(2): 100391Read more>>
  • Prema Robinson. et al. Therapeutic Potential of a Small-Molecule STAT3 Inhibitor in a Mouse Model of Colitis. CANCERS. 2023 Jan;15(11):2977Read more>>
  • Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
  • Nahyun Choi. et al. Involvement of DKK1 secreted from adipose-derived stem cells in alopecia areata. CELL PROLIFERAT. 2023 Nov;:e13562Read more>>
  • Kasem Shaimaa M.. et al. Prophylactic and Therapeutic Efficacy of Ultrasonicated Rosmarinus officinalis Ethanolic Extract and its Chitosan-Loaded Nanoparticles Against Eimeria tenella Infected Broiler Chickens. ACTA PARASITOL. 2024 Mar;:1-49Read more>>
VALIDATION IMAGES

Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with (bs-0647R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\n


Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.


Image provided by the Independent Validation Program, badge number 029634:Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, AF555 conjugated (bs-0647R-A555) at 1:200 at 4°C for 90 minutes and DAPI staining of the nuclei.


Lane 1: HL60 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Spleen; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Lymph Node; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human U937 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Jurkat cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD4 Monoclonal Antibody(bs-0647R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.