bs-0654R [Primary Antibody]
GHR Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GHR

Immunogen Range: 101-200/451


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2690

Swiss Prot: P10912

Source: KLH conjugated synthetic peptide derived from human GHR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse
Rat
Pig
Sheep
Others

Predicted Cross Reactive Species: Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Hai Li, et al. "Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte." Molecular biology reports 39.9 (2012): 9113-9122.Read more>>
  • Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.Read more>>
  • Hetz, Jennifer A., et al. "Growth axis maturation is linked to nutrition, growth and developmental rate." Molecular and Cellular Endocrinology (2015).Read more>>
  • Song, Cheng-Jun, et al. "Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes." International Journal of Clinical and Experimental Medicine 8.7 (2015): 10411-10419.Read more>>
  • Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).Read more>>
  • Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).Read more>>
  • Zhou et al. Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets. (2018) Asian-Australas.J.Anim.Sci. 31:32-39Read more>>
  • Chen et al. Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. (2013) Neural.Regen.Res. 8:1756-64Read more>>
  • Cui et al. Generation of a miniature pig disease model for human Laron syndrome. (2015) Sci.Rep. 5:15603Read more>>
  • Xie W et al. Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-likegrowth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus). Gen Comp Endocrinol. 2019 May 20;281:58-66. Read more>>
  • Zhou M et al. Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate. J Anim Physiol Anim Nutr (Berl). 2019 Sep 4. Read more>>
  • Fengyang Wu. et al. Effects of zearalenone on ovarian development of prepubertal gilts through growth hormone axis. FRONT VET SCI. 2022; 9: 950063Read more>>
  • Ahmed O. Kaseb. et al. Blockade of growth hormone receptor signaling by using pegvisomant: A functional therapeutic strategy in hepatocellular carcinoma.. FRONT ONCOL. 2022 Oct;12:986305-986305Read more>>
  • Hu Bowen. et al. Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-18Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining


Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).


This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:300 followed by conjugation to a secondary antibody and staining.


Lane 1: Mouse Testis lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Testis lysates; Lane 4: Rat Cerebrum lysates probed with GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.